AIDS-RELATED MYCOBACTERIAL DISEASE--ANTIBODIES TO MYCOBACTERIUM AVIUM IN AIDS SER

美国约50%的艾滋病患者患上MAC 疾病随着疾病的发展而发展。我们已经开始评估免疫力 艾滋病感染者对MAC抗原的反应。我们已经化验了 检测20例艾滋病合并MAC病患者血清中的抗MAC抗体。 非艾滋病患者和10例健康对照。 西方分析表明艾滋病和非艾滋病患者患有MAC 感染对禽类分枝杆菌有一种有限的、异质的体液反应。 尽管对禽类分枝杆菌的特异性抗体反应具有很高的变异性， 识别30、37或47 kDa MAC蛋白的抗体已经被 在其中约60%的患者中发现了这种病毒。抗30和37 kDa抗体 在相当大比例的对照血清中也存在蛋白质。 然而，识别47 kDa MAC蛋白的抗MAC抗体还没有 在对照血清中发现。进一步分析了大量的 将需要对照血清来确定血清学检测是否 对47 kDa MAC抗原的体液反应将进行量化 对活动期MAC疾病的诊断。 我们还用含有8个蛋白质的免疫印迹法培养了艾滋病血清 重组MAC蛋白。我们所有的艾滋病血清库都会产生强烈的反应 含有34 kDa的分枝杆菌蛋白。我们目前正在刻画 确定这种蛋白质及其基因的意义。

Approximately 50% of patients with AIDS in the United States develop MAC disease as the disease progresses. We have begun evaluating the immune response to MAC antigens of AIDS-infected individuals. We have assayed for anti-MAC antibodies the sera of 20 AIDS patients with MAC disease, 5 non-AIDS patients with M. avium disease and 10 healthy controls. Western analysis indicates AIDS and non-AIDS patients with MAC infections have a limited, heterogeneous humoral response to M. avium. Although the specific antibody response to M. avium is highly variable, antibodies recognizing 30, 37 or 47 kDa MAC proteins have been identified in about 60% of these patients. Antibodies to 30 and 37 kDa proteins are also present in a significant proportion of control sera. However, anti-MAC antibodies recognizing the 47 kDa MAC protein have not been seen in control sera. Further analysis of a large number of control sera will be required to ascertain whether serological assays quantitating humoral responses to the 47 kDa MAC antigen will be diagnostic for active MAC disease. We have also incubated the AIDS sera with Western blots containing 8 recombinant MAC proteins. All of our AIDS serum pools react strongly with a 34 kDa mycobacterial protein. We currently are characterizing this protein and its gene to determine its significance.