ANALYSIS OF RAF-ONCOGENE RELATED NOVEL PROTEIN KINASE GENE

我们继续分析一种新的蛋白激酶基因，编码一种 与RAF癌基因具有广泛同源性的蛋白质。三种不同的 已克隆的各版本的cdna都具有保守的氨基端。 结尾，但催化结构域不同，表明不同 在底物特性方面。氨基末端含有一个不同寻常的复制 具有类似于核定位信号或DNA的特性的序列 结合域。目前，我们正在测试针对羧基的抗体。 用来定位该激酶的细胞内位置的末端多肽 以测定体外激活酶活性。我们还在测试激酶基因 在NIH 3T3成纤维细胞转化中的转化能力 化验。

We have continued our analysis of a novel protein kinase gene encoding a protein with extensive homology to the raf oncogene. Three different versions of the cDNA have been cloned all with conserved amino-terminal ends, but with differences in the catalytic domains, suggesting differences in substrate specificities. The amino end contains an unusual duplicated sequence with properties similar to nuclear localisation signals or DNA binding domains. Currently, we are testing antibodies made to carboxy terminal peptides to localize the intracellular position of the kinase and to assay for in vitro kinase activity. We are also testing the kinase gene for transforming capabilities in the NIH 3T3 fibroblast transformation assay.