AMINOACYL-TRNAS IN HIV AND OTHER RETROVIRAL INFECTED CELLS

我们在过去一年中的注意力集中在具体的 在核糖体移码内的A位点利用的tRNA 逆转录病毒信号。猴获得性免疫缺陷病毒 综合征[SAIDS]）含有前pol移码信号A AAU UUU，其中 苯丙氨酸tRNA在核糖体A位点翻译UUU密码子， 天冬酰胺tRNA前阅读中核糖体P位点的AAU密码子 frame.在SRV-1感染的细胞中，发现苯丙氨酸tRNA缺乏 在其反密码子环中高度修饰Wye碱基，而天冬酰胺tRNA 在其反密码子环中缺乏高度修饰的Q碱基。这些结果 进一步证实了早期观察到的与tRNA 低修饰和对相应的异源受体的需要， 翻译HIV，HTLV-1核糖体移码信号内的密码子 和BLV。此外，赖氨酸tRNA 4在SRV-1感染的细胞中升高， 该同种受体用作逆转录酶的引物。我们 他们还开始研究HIV病毒中的tRNA群体， 并开发了一种体内试验，用于检查 核糖体移码中的低修饰tRNA。

Our attention in the past year has focused on the role of the specific tRNAs which are utilized at the A-site within the ribosomal frameshift signal in retroviruses. SRV-1 (simian acquired immunodeficiency virus syndrome [SAIDS]) contains the pro-pol frameshift signal A AAU UUU where phenylalanine tRNA translates the UUU codon at the ribosomal A-site and asparagine tRNA the AAU codon at the ribosomal P-site in the pro reading frame. In SRV-1 infected cells, phenylalanine tRNA was found to lack the highly modified Wye base in its anticodon loop, while asparagine tRNA lacked the highly modified Q base in its anticodon loop. These results further substantiate an earlier observation in correlating tRNA hypomodification and the requirement for the corresponding isoacceptor in translating codons within the ribosomal frameshift signals of HIV, HTLV-1 and BLV. In addition, lysine tRNA4 was elevated in SRV-1 infected cells and this isoacceptor is utilized as primer for the reverse transcriptase. We have also begun a study of the tRNA population included within the HIV virion and have developed an in vivo assay for examining the role of hypomodified tRNAs in ribosomal frameshifting.