BIOCHEMICAL CYTOLOGY OF HOST-PARASITE INTERACTIONS IN PARASITIC PROTOZOA

利什曼原虫的细胞生物学和生物化学研究如下 一种细胞内和细胞外寄生的模型。AS 寄主和寄生虫之间的相互作用发生在 寄生虫表面膜(SM)，重点放在：1) 描述其生化和生理功能；2) 明确了它在寄生虫生存和发展中的作用。 杜氏乳杆菌前鞭毛体的SM-Pro运输蛋白是 使用定向亲和矩阵结合和反-亲和矩阵结合进行鉴定 以独特型抗体(抗-ID)为基础的方法。反ID是 针对亲和纯化的抗多聚脯氨酸抗体产生。这个 抗ID免疫共沉淀法检测利什曼原虫SM蛋白及其 抑制Pro转运到完整的前鞭毛体中 超过50%。SM酸性磷酸酶得到部分纯化 并以此为特征。青蒿素的生物合成和分泌机制 对可溶性酸性磷酸酶(SACP)进行了定义和定位。 在SACP之间显示了抗原的交叉反应 由各种利什曼物种的前鞭毛体产生的。 用寡核苷酸探针鉴定和克隆该基因(S) 用于SACP。将SM 3‘-核苷酸酶纯化为均一酶。 其氨基末端序列。SM 5‘-核苷酸酶也 分离、纯化和鉴定。 目前的结果提供了进一步的功能表征 寄生的SM成分，这些成分可能是 设计新的化疗药物和/或免疫诊断/ 预防性试剂。

The cell biology and biochemistry of Leishmania is investigated as a model of both intra- and extra-cellular parasitism. As interactions between host and parasite occur at the level of the parasite surface membrane (SM), emphasis is placed on: 1) characterizing its biochemical and physiological functions; and 2) defining its roles in parasite survival and development. The SM proline-transport protein of L. donovani promastigotes was identified using both directed affinity matrix-binding and anti- idiotype antibody (anti-ID)-based methods. The anti-ID was generated against an affinity purified anti-polyproline IgG. The anti-ID immunoprecipitated the Leishmania SM protein and its also inhibited proline transport into intact promastigotes by greater than 50 percent. The SM acid phosphatase was partially purified and characterized. The biosynthetic and secretory mechanisms of the soluble acid phosphatase (SAcP) were defined and localized. Antigenic cross-reactivity was demonstrated amongst the SAcP's produced by promastigotes of various leishmanial species. Oligonucleotide probes were used to identify and clone the gene(s) for SAcP. The SM 3'-nucleotidase was purified to homogeneity and its amino terminus sequences. The SM 5'-nucleotidase was also isolated, purified and characterized. The current results provide further functional characterization of parasite SM constituents and these might serve as targets for the design of new chemotherapeutic agents and/or immunodiagnostic/ prophylactic reagents.