ANTIGENIC MAPPING OF CHLAMYDIAL PROTEINS

沙眼衣原体主要外膜蛋白(MOMP) 已被确定为一种可能的疫苗免疫原 发展。这个项目的目标是确定氨基 表面可接触的MOMP的酸序列 并确定这些序列是否 在感染期间以免疫为主。含有以下序列的多肽 四个可变区(VDI、VDII、VDIII和VDIV)已经被 合成的。这些多肽已被用来定义表位 单抗与MOMP的结合部位，特别是以下单抗的结合部位 已知被动地投射以抵抗猴眼或 在小鼠毒性试验中。感染过程中的抗体反应 对鹦鹉热杆菌GPIC菌株的VDS进行检测 GPIC四种VDS的合成肽及检测血清和 感染和免疫的豚鼠的眼泪。多克隆兔 抗血清被提高到A、E和L2 MOMP的VDIII以确定 如果这种Vd是表面可达的，并检查其同源性 血清型中的VDIII。胰酶对MOMP表面的裂解作用 活的EBS导致EBS与HeLa 229细胞失去结合。 只有当B MOMP在VDII和VDII中都被切割时，才会发生结合丢失 VDIV。提示MOMP在衣原体相互作用中的作用 与宿主细胞。VDII和VDIV对应的合成肽 的B和L2血清型已经合成，并将用于 确定它们在衣原体与HeLa 229细胞结合中的作用。

The major outer membrane protein (MOMP) of Chlamydia trachomatis has been identified as a possible immunogen for vaccine development. The objectives of this project are to identify amino acid sequences of MOMP which are surface accessible to immunoglobulins and to determine if these sequences are immunodominant during infection. Peptides containing sequences of the four variable domains (VDI, VDII, VDIII, and VDIV) have been synthesized. These peptides have been used to define the epitope binding site of MAbs to MOMP, in particular, of MAbs which are known to passively project against infection in the monkey eye or in the mouse toxicity test. The antibody response during infection to the VDs of C. psittaci strain GPIC will be tested using synthetic peptides of all four VDs of GPIC and testing sera and tears from infected and immune guinea pigs. Polyclonal rabbit antisera is being raised to VDIII of A, E, and L2 MOMP to determine if this VD is surface accessible and to examine the homology of VDIII among serovars. Trypsin cleavage of MOMP at the surface of viable EBs results in loss of binding of EBs to HeLa 229 cells. Loss of binding occurs only when B MOMP is cleaved in both VDII and VDIV. suggesting a role for MOMP in interactions of chlamydiae with host cells. Synthetic peptides corresponding to VDII and VDIV of B and L2 serovars have been synthesized and will be used to determine their role in binding of chlamydiae to HeLa 229 cells.