EFFECT OF ALCOHOL ON THE HUMORAL IMMUNE RESPONSE

酒精对细胞免疫和 免疫反应和体液免疫反应。 动物和人类 摄入酒精会降低对新冠病毒的抗体反应， 抗原 酒精抑制抗体的机制 形成是未知的。 主要研究者将使用 抗体形成的体外模型，以测试假设， 酒精通过干扰 生物化学途径负责启动和维持 B和T淋巴细胞中免疫应答。 她会用独一无二的 DNP特异性B的长期连续非恶性细胞系 淋巴细胞在她的实验室和KLH特异性T 辅助淋巴细胞，以确定酒精是否直接影响B TH细胞。 这些细胞系将用于确定 B细胞和T细胞反应的哪个阶段被酒精抑制。 在确定免疫反应的时间后， 抑制发生时，将使用相同的体外系统来 比较磷脂酰肌醇途径、环磷酸腺苷 水平和Ca+2内流和动员，发生在 抑制点是否存在酒精。 44Kd蛋白的磷脂甲基化和磷酸化 也将审查是否压制似乎涉及行动， B细胞刺激因子1或B细胞分化因子μ. 与急性酒精暴露抑制相关的变化 然后将其与慢性酒精引起的变化进行比较 exposure. 慢性酒精暴露将在两个 在体内通过喂养酒精小鼠和使用脾细胞， 在酒精的存在下培养细胞系。

Alcohol has a suppressive effect on both the cellular immune response and humoral immune response. Animals and humans who have ingested alcohol have a decrease in antibody response to new antigens. The mechanisms by which alcohol suppresses antibody formation are not known. The principal investigator will use an in vitro model of antibody formation to test the hypothesis that alcohol inhibits the antibody response by interfering with biochemical pathways responsible for initiating and sustaining the immune response in B and T lymphocytes. She will use the unique long term continuous non malignant cell lines of DNP specific B lymphocytes developed in her laboratory and KLH specific T helper lymphocytes to determine if alcohol directly effects both B and TH cells. These cell lines will then be used to determine which stage of B cell and T cell response is inhibited by alcohol. After determining the time in the immune response when suppression occurs, the same in vitro systems will be used to compare the changes in phosphotidyl inositol pathway, cyclic AMP levels and Ca+2 influx and mobilization which occur in the presence and absence of alcohol at the point of suppression. Phospholipid methylation and phosphorylation of a 44 Kd protein will also be examined if suppression appears to involve the action of B cell stimulation Factor 1 or B cell differentiation factor mu. Changes associated with suppression by acute alcohol exposure will then be compared to changes induced by chronic alcohol exposure. Chronic alcohol exposure will be accomplished both in vivo by feeding alcohol mice and using spleen cells, and in vitro by growing the cell lines in the presence of alcohol.