EFFECT OF ALCOHOL ON THE HUMORAL IMMUNE RESPONSE

酒精对体液免疫反应的影响

• 批准号: 3111248
• 负责人: MARLENE A. ALDO-BENSON
• 金额: $ 12.7万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-28 至 1990-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3111248
• 关键词: B lymphocyte; T lymphocyte; adenosine triphosphate; adenylate cyclase; alcoholic beverage consumption; alcoholism /alcohol abuse; amiloride; antibody formation; biological fluid transport; calcium; calcium channel blockers; carbonates; computer data analysis; cyclic AMP; cystic fibrosis; cytotoxicity; electrolyte balance; electrophysiology; embryo /fetus culture; gel electrophoresis; gestational age; helper T lymphocyte; high performance liquid chromatography; histogenesis; human subject; human tissue; humoral immunity; immunosuppression; leukocyte activation /transformation; ligands; magnesium; mammalian embryology; membrane permeability; membrane potentials; methylation; phosphatidylinositols; phospholipids; phosphorylation; plaque assay; polymers; protein kinase; radioimmunoassay; respiratory epithelium; scintillation counter; thin layer chromatography; tissue /cell culture; trachea

Alcohol has a suppressive effect on both the cellular immune response and humoral immune response. Animals and humans who have ingested alcohol have a decrease in antibody response to new antigens. The mechanisms by which alcohol suppresses antibody formation are not known. The principal investigator will use an in vitro model of antibody formation to test the hypothesis that alcohol inhibits the antibody response by interfering with biochemical pathways responsible for initiating and sustaining the immune response in B and T lymphocytes. She will use the unique long term continuous non malignant cell lines of DNP specific B lymphocytes developed in her laboratory and KLH specific T helper lymphocytes to determine if alcohol directly effects both B and TH cells. These cell lines will then be used to determine which stage of B cell and T cell response is inhibited by alcohol. After determining the time in the immune response when suppression occurs, the same in vitro systems will be used to compare the changes in phosphotidyl inositol pathway, cyclic AMP levels and Ca+2 influx and mobilization which occur in the presence and absence of alcohol at the point of suppression. Phospholipid methylation and phosphorylation of a 44 Kd protein will also be examined if suppression appears to involve the action of B cell stimulation Factor 1 or B cell differentiation factor mu. Changes associated with suppression by acute alcohol exposure will then be compared to changes induced by chronic alcohol exposure. Chronic alcohol exposure will be accomplished both in vivo by feeding alcohol mice and using spleen cells, and in vitro by growing the cell lines in the presence of alcohol.

酒精对细胞免疫既有抑制作用 免疫应答和体液免疫应答。动物和人类 是否摄入酒精会降低对新病毒的抗体反应 抗原。酒精抑制抗体的机制 编队情况尚不清楚。首席调查员将使用In 抗体形成的体外模型，以检验这一假设 酒精通过干扰抗体反应抑制抗体反应 负责启动和维持细胞周期的生化途径 B和T淋巴细胞的免疫反应。她将使用唯一的 DNP特异性B的长期连续非恶性细胞系 她实验室中发育的淋巴细胞和KLH特异性T细胞 辅助性淋巴细胞确定酒精是否直接影响两种B细胞 和TH细胞。然后这些细胞系将被用来确定 酒精抑制B细胞和T细胞反应的哪个阶段。 在确定了免疫反应中的时间后 抑制发生时，相同的体外系统将被用于 比较磷脂酰肌醇途径、环磷酸腺苷的变化 水平和Ca+2内流和动员发生在 抑制点酒精的存在和不存在。 44kD蛋白的磷脂甲基化和磷酸化 如果抑制似乎涉及该操作，也将进行检查 B细胞刺激因子1或B细胞分化因子Mu。 与急性酒精暴露抑制相关的变化 将其与长期饮酒引起的变化进行比较 曝光。慢性酒精暴露将在两个月内完成 通过喂饲酒精小鼠和使用脾细胞进行体内实验，并在体外通过 在酒精存在的情况下培养细胞系。