BIOLOGICAL AND BIOCHEMICAL STUDIES OF ANTIGENS ON MALARIA-INFECTED RED CELLS

我们正在研究疟疾蛋白的结构和功能。 插入并穿过感染的红细胞膜 带有成熟的无性疟疾寄生虫和分子 内皮细胞参与细胞黏附。 五种疟疾蛋白已定位于 成熟期感染恶性疟原虫的红细胞 滋养体阶段。MR大约30万个细胞表面 与细胞黏附特性相关的蛋白质 内皮细胞(记为Pf EMP 1、恶性疟原虫红细胞 膜蛋白1)已被证明具有独特的抗原性。 来自另一个非常大的(约300,000)疟疾 蛋白质位于表面膜下的电子致密 旋钮上的材料(表示为PF EMP 2)。富含组氨酸的蛋白质 (HRP)与旋钮表达相关(标记为PfHRP 1) 也被证明定位在膜下的电子- 致密的材料。从受感染细胞输出的另一种HRP 进入血浆(称为Pf HRP 2)也与表面有关 膜，推测是在分泌的途中，因为它是 在细胞表面可被抗体访问，也可附着在 细胞骨架。我们还展示了RESA，一种插入 在裂殖子入侵期间，宿主膜保留在 膜，并可被乳过氧化物酶催化 滋养体感染细胞的放射性碘标记。

We are studying the structure and function of malarial proteins inserted into and through the membrane of erythrocytes infected with mature asexual malaria parasites and molecules on the endothelial cells involved in cytoadherence. Five malarial proteins have been localized to the membrane of Plasmodium falciparum-infected erythrocytes at the mature trophozoite stage. The Mr approximately 300,000 cell surface protein associated with the property of cytoadherence to endothelial cells (denoted Pf EMP 1, P. falciparum erythrocyte membrane protein 1) has been shown to be antigenically distinct from another very large (Mr approximately 300,000) malarial protein located under the surface membrane in the electron-dense material at knobs (denoted Pf EMP 2). The histidine-rich protein (HRP) associated with knob expression (denoted PfHRP 1) was also shown to be localized under the membrane in the electron- dense material. Another HRP that is exported from infected cells into plasma (called Pf HRP 2) is also associated with the surface membrane, presumably en route for secretion since it is accessible to antibody at the cell surface and also attached to the cytoskeleton. We also showed that RESA, a protein inserted into the host membrane during merozoite invasion, remains in the membrane and is accessible to lactoperoxidase catalyzed radioiodination with trophozoite-infected cells.