STRUCTURE AND FUNCTION OF THE ADV GENOME

该项目的目的是研究基因组结构， 水貂细小病毒阿留申病（ADV）的功能。 在 去年，我们完成了ADV-G基因组的测序， 发现它的整体结构类似于其他 无缺陷细小病毒。 一部分“左手”打开 阅读框（ORF）已在细菌中表达，并显示出 ADV的71，000道尔顿非结构蛋白的代码。 结构蛋白区的序列异质性 ADV基因组具有异质性 观察到的可能反映了涉及宿主范围的决定因素， 致病性 最后，研究ADV转录程序 已经开始了。 三种主要的多聚腺苷酸化RNA已经被 检测到; 4.5 kb、2.6 kb和0.9 kb。

The purpose of this project is the study of genome structure and function of the Aleutian disease of mink parvovirus (ADV). In the past year we have completed sequencing of ADV-G genome and found that its overall structure resembles that of other nondefective parvoviruses. A portion of the "left-hand" open reading frame (ORF) has been expressed in bacteria and shown to code for the 71,000 dalton nonstructural protein of ADV. Sequence heterogeneity for the structural protein region of the ADV genome has been demonstrated with the heterogeneity observed might reflect host range determinants involved with pathogenicity. Finally, study of the ADV transcription program has been initiated. Three major polyadenylated RNAs have been detected; 4.5 kb, 2.6 kb, and 0.9 kb.