IMMUNOBIOLOGY OF GUINEA PIG INCLUSION CONJUNCTIVITIS

复发性眼衣原体感染的发病机制是 被认为是免疫调节的(即，超敏反应)。 我们已经建立了一个动物模型来研究免疫学 在感染和挑战期间对衣原体抗原的反应。 我们选择研究豚鼠的免疫病理学。 内涵性结膜炎(GPIC)，鹦鹉热衣原体 会引起豚鼠的眼部和生殖器感染。我们 具有临床、微生物学和组织学特征 原发GPIC和迟发性超敏反应 衣原体抗原。出现迟发性超敏反应 仅限于以前在黏膜表面感染过GPIC的动物 探员。衣原体超敏反应抗原已经被 鉴定为多肽，MR为57kD。我们有 利用豚鼠动物模型，建立了一种角膜模型 混浊和血管化。青霉素在初诊期间的应用 GPIC阻止衣原体在网状细胞中的发育周期 处于身体阶段，从而防止感染性颗粒的发展。 这些网状体保持在 宿主细胞和分泌衣原体抗原，这可能导致更多 严重结膜炎和角膜通过宿主受累 免疫机制。这可能会被证明是一个有用的研究模型。 导致沙眼失明的并发症。我们是 探讨迟发型超敏反应在输卵管炎和输卵管炎中的作用 采用豚鼠输卵管性不孕症模型。初步结果 提示有原发性阴道或输卵管感染、水肿症 和细胞浸润类似于延迟的 受刺激时，输卵管会出现过敏反应 使用GPIC的Triton X-100萃取物。这种动物模型 衣原体引起的不孕症正在进一步发展。

The pathogenesis of recurrent ocular chlamydial infections is thought to be immunologically mediated (i.e., hypersensitivity). We have developed an animal model to study the immunological response to chlamydial antigens during infection and challenge. We have chosen to study the immunopathology of guinea pig inclusion conjunctivitis (GPIC), a Chlamydia psittaci strain which produces both ocular and genital infections in guinea pigs. We have clinically, microbiologically, and histologically characterized primary GPIC and a delayed hypersensitivity response to a chlamydial antigen. The delayed hypersensitivity response occurs only in animals previously infected at a mucosal surface by GPIC agent. The chlamydial hypersensitivity antigen has been identified as a polypeptide with an Mr of 57 kD. We have developed, using the guinea pig animal model, a model for corneal opacity and vascularization. Penicillin treatment during primary GPIC arrest the chlamydial developmental cycle at the reticulate body stage and thus prevents development of infectious particles. These reticulate bodies are maintained within inclusions in the host cell and secrete chlamydial antigens which may lead to more severe conjunctivitis and corneal involvement through host immune mechanisms. This may prove a useful model to study complications leading to blindness in trachoma. We are investigating the role of delayed hypersensitivity in salpingitis and tubal infertility using the guinea pig model. Preliminary results indicate following a primary vaginal or oviductal infection, edema and cellular infiltration similar to that seen in a delayed hypersensitivity response occurs in the oviduct upon challenge with the Triton X-100 extract of GPIC. This animal model of chlamydial caused infertility is being further developed.