IMMUNOCHEMICAL STUDIES OF ACTINOMYCES VISCOSUS POLYSACCHARIDES

粘性放线菌产生的多糖可以促进 这些微生物粘附于吸附在 牙釉质或暴露的牙根表面。 它们可以抑制或增强 凝集素介导的口腔细菌之间的共聚集，和 导致牙菌斑大量形成。 这些多糖还可以 诱导抗体形成， 龋齿的发展。 虽然A.粘性产生 几种多糖，它们的化学精细结构仍然存在， 被阐明。 评价它们在根病发病中的作用 表面龋齿也缺乏，因此，该提案旨在： 1-在完整的A.粘液细胞定量 荧光染料标记的凝集素结合试验， 结合位点的性质、数量和亲和力（Ka）; 2-以纯化形式分离细胞壁、细胞壁缔合物和 T6、T14 V和T14 Av菌株的胞外多糖 A.粘度和表征其分子结构， 化学和免疫化学方法; 3-为了检测分离的多糖作为 抗原抑制细菌附着到唾液包被的 羟基磷灰石包覆的珠粒并抑制细菌共聚集; 4-检测大鼠抗体结合位点特异性， 兔抗A.粘多糖 抗原; 5-用分离的多糖抗原主动免疫大鼠 以确定特定聚合物刺激 抑制或增强口腔感染后疾病， 6-制备抗分离的 多糖抗原，阐明其结合位点的特异性 并评估特异性抗碳水化合物在修饰 在无特定病原体的大鼠中的植入和疾病。

Polysaccharides produced by Actinomyces viscosus may promote adherence of those microorganisms to glycoproteins adsorbed to enamel or exposed root-surfaces. They may inhibit or enhance lectin mediated coaggregation between oral bacteria, and contribute to the bulk of plaque. These polysaccharides can also induce formation of antibodies that may hinder or promote development of dental caries. Although A. viscosus produces several polysaccharides their chemical fine structure remains to be elucidated. Evaluation of their role in the pathogenesis of root surface caries is also lacking, This proposal therefore aims: 1-on the surface of intact A. viscosus cells by quantitative fluorochrome-tagged lectin binding assays which yield information on the nature, number and affinity (Ka) of binding sites; 2- to isolate in purified form, cell wall, cell wall associated and extracellular polysaccharides from T6, T14V and T14Av strains of A. viscosus and characterize their molecular structure by chemical and immunochemical methods; 3- to examine the ability of isolated polysaccharides to serve as antigen inhibit attachment of bacteria to saliva coated hydroxylapatite coated beads and inhibit bacterial coaggregation; 4- to examine the antibody combining site specificity of rat and rabbit polyclonal antibody to isolate A. viscosus polysaccharide antigens; 5- to actively immunize rats with isolated polysaccharide antigens to determine the ability of specific polymers to stimulate suppression or enhancement of disease upon oral infection and 6- to prepare monoclonal rat antibodies against isolated polysaccharide antigens, elucidated their combing site specificity and evaluate the role of specific anti-carbohydrate in modifying implantation and disease in specific pathogen-free rats.