TRANSFORMATION BY HUMAN CMV AND HHV-6

人巨细胞病毒（HCMV）与几种肿瘤相关， 疾病，包括前列腺癌，结肠腺癌， 宫颈癌和卡波西肉瘤 形态转换 CMV Towne的II区（mtrII）先前已定位于980 含有三个推定的开放阅读框架（ORF）的碱基对片段 79、83和34个氨基酸（aa）。 同源区存在于菌株中 Towne，AD 169和Tanaka。 然而，田中mtrII减少了转换 与Towne和AD 169相比， 有趣的是，79个氨基酸 存在于Towne和AD 169中的酸性ORF在Tanaka中被截短， 这表明它可能对有效转化至关重要。 两个功能性启动子（P1和P2）已在Towne HCMV mtrII。该79个氨基酸的ORF可以从P1 转录本，而83和34个氨基酸的ORF可以从 或是成绩单。 检测到类似的功能启动子活性 在来自HCMV的mtrII共线转化和非转化区域内 菌株AD 169和Tanaka。 来自菌株的启动子 汤恩表现出了改造活动。 结合此前数据， 这些数据表明，HCMV mtrII的转化不会发生， 启动子插入，但需要完整的79个氨基酸的ORF。

Human cytomegalovirus (HCMV) has been associated with several neoplastic diseases, including prostatic carcinoma, adenocarcinoma of the colon, cervical carcinoma, and Kaposi's sarcoma. The morphological transforming region II (mtrII) of CMV Towne has previously been localized to a 980 base-pair fragment containing three putative open reading frames (ORFs) of 79, 83, and 34 amino acids (aa). Homologous regions exist in strains Towne, AD169 and Tanaka. However, Tanaka mtrII has reduced transforming activity when compared to Towne and AD169. Interestingly, the 79 amino acid ORF present in both Towne and AD169 is truncated in Tanaka, indicating that it may be crucial for efficient transformation. Two functional promoters (P1 and P2) have been mapped within the Towne HCMV mtrII. The 79 amino acid ORF can be translated from the P1 transcript, while the 83 and 34 amino acid ORFs could be translated from either transcript. Similar functional promoter activities were detected within mtrII colinear transforming and non-transforming regions from HCMV strains AD169 and Tanaka, respectively. Neither promoter from strain Towne exhibited transforming activity. Combined with previous data, these data suggest that transformation by HCMV mtrII does not occur by promoter insertion but requires an intact 79 amino acid ORF.