MOLECULAR APPROACHES FOR DEVELOPMENT OF AN HIV VACCINE--RHESUS MACAQUES MODEL

这个项目是去年的延续。它的目的是在 恒河猴对携带人类的各种活载体的疗效 免疫缺陷病毒1型(HIV-1)、HIV-2和猴免疫缺陷 病毒(SIV)抗原在诱导保护性免疫反应中的作用。我们是 测试三种不同的递送系统：1)减毒牛痘和 携带HIV和SIV抗原的禽痘；以及2)减毒沙门氏菌 鼠伤寒沙门氏菌作为细菌疫苗，携带HIV/SIV抗原。我们 还计划进一步刺激患者的免疫反应 使用纯化的病毒抗原(天然或 重组病毒亚基)。我们将使用恒河猴，这是 容易感染SIV(Mac251)和HIV-2(SBL/ISY)。这个 我们研究的终点将是防止感染任何一种 SIV或HIV-2。含有HIV-1抗原的载体也将被评估 它们在猕猴体内的免疫原性。免疫的动物将是 在接下来的几个月里受到了挑战。

This project is a continuation from last year. Its aim is to test in rhesus macaques the efficacy of various live vectors carrying human immunodeficiency virus type-1 (HIV-1), HIV-2 and simian immunodeficiency virus (SIV) antigens in eliciting a protective immunoresponse. We are testing three different delivery systems: 1) attenuated vaccinia and avipox carrying HIV and SIV antigens; and 2) attenuated Salmonella typhimurium strain as a bacterial vaccine, carrying HIV/SIV antigens. We are also planning to further stimulate the immunoresponse in the immunized animals using purified viral antigens (either native or recombinant viral subunits). We will use rhesus macaques which are susceptible to the infection of both SIV(mac251) and HIV-2(SBL/ISY). The end point of our studies will be the protection from infection by either SIV or HIV-2. Vectors containing HIV-1 antigens will also be evaluated for their immunogenicity in rhesus macaques. The immune animals will be challenged in the next few months.