GENETIC ANALYSIS OF HUMAN CELLULAR GENES IN NEOPLASTIC TRANSFORMATION

人类基因图谱计划保持着持续的合作 与校内和校外实验室的互动。 这 该项目利用三个原则进行物理和遗传绘图， 技术. 快速增殖的人乳腺癌细胞的Southern印迹分析 X啮齿动物体细胞杂种DNA用于基因定位 特定染色体的序列。 第二，原位荧光 染色体杂交取代了同位素杂交， 提供靶序列的精确区域定位， 的背景。 最后，对多态性进行分离或连锁分析， 基因或DNA标记进行使用中心D '研究杜 多态性Humain（CEPH）参考家族，包含约50个 三代血统 这些方法导致了 一些细胞因子，G蛋白偶联受体，细胞因子， 癌基因、新型酪氨酸激酶和调节DNA结合蛋白。 此外，正在进行一项研究，以建立一个高分辨率的链接， 和3号染色体的细胞遗传学图谱。 最终，这些地图将导致 肿瘤抑制基因的分离和鉴定 家族性和非家族性癌症，以及确定折点 某些疾病中的染色体重排。 校长 他是人类基因组计划的研究员，也是 国际比较基因图谱委员会

The Human Gene Mapping Program maintains an ongoing collaborative interaction with both intramural and extramural laboratories. This project carries out physical and genetic mapping using three principle technologies. Southern blotting analysis of rapidly proliferating human X rodent somatic cell hybrid DNA is used for the assignment of gene sequences to specific chromosomes. Secondly, fluorescence in situ chromosome hybridization, which has replaced isotopic hybridization, provides precise regional localization of target sequences with a minimum of background. Finally, segregation or linkage analysis of polymorphic genes or DNA markers is carried out using the Centre D'Etude du Polymorphisme Humain (CEPH) reference families which contain about 50 three-generation pedigrees. These methodologies have resulted in the assignment of a number of cytokines, G-protein coupled receptors, cellular oncogenes, novel tyrosine kinases, and regulatory DNA binding proteins. In addition, a study is underway to construct a high resolution linkage and cytogenetic map of chromosome 3. Ultimately, these maps will lead to the isolation and characterization of tumor suppressor genes involved in both familial and non-familial cancer and in determining the breakpoints of chromosomal rearrangements in certain diseases. The principal investigator serves in the human genome initiative as well as chairman of the International Committee on Comparative Gene Mapping.