USE OF IMMUNOLOGICAL TECHNIQUES TO STUDY THE INTERACTION OF CARCINOGENS WITH DNA

针对致癌物-DNA加合物的抗体已被用于量化 多环取代生物样品中的DNA修饰 芳香烃、芳香胺和顺铂的定量测定 免疫分析、免疫组化、免疫亲和层析(IAC)、 原子吸收光谱(AAS)和P-32-后标记。研究是 焦炉血细胞DNA中多环芳烃DNA加合物的测定 工人，铝厂工人，食用炭烧牛肉和 暴露在科威特油井大火中的军队人员，使用 苯并[a]芘-DNA酶联免疫吸附试验和6- 倍增灵敏解离增强型稀土元素荧光免疫分析 (DELFIA)芳香胺-DNA加合物正从人类体内浓缩 IAC法检测肺DNA，P-32-后标记法定量。其影响范围 肿瘤有核血细胞DNA中顺铂-DNA加合物的形成 患者(通过顺铂-DNA酶联免疫吸附试验测量)呈正相关 接受铂治疗的卵巢癌患者的疾病反应 化疗。目前正在进一步研究血液中的这种联系。 来自以前未经治疗的卵巢癌患者的样本，都给出了 相同的协议。已在胎盘和胎盘中测量到铂-DNA加合物 一名接受顺铂和卡铂治疗卵巢的妇女的血细胞DNA 怀孕期间罹患癌症。这种人类接触是在帕塔斯中模拟的 在母体组织和胎儿组织中均可检测到加合物。 血细胞铂-DNA加合物与剂量相关 3个周期睾丸癌患者标本的ELISA法和AAS法检测 铂类药物化疗。一种抗艾滋病的特异性抗血清 药物3‘-叠氮-2’，3‘-二脱氧胸苷(AZT)已被用于建立 AZT掺入的放射免疫分析和免疫组织化学定位 人HL60、中国仓鼠卵巢(CHO)和小鼠NIH3T3的DNA 细胞系。放射性标记相关法将AZT掺入DNA 很好，这是由RIA确定的。AZT的最高浓度 掺入CHO细胞染色体DNA与DNA区域共定位 在Z构象中。

Antibodies specific for carcinogen-DNA adducts have been used to quantify DNA modification in biological samples substituted with polycyclic aromatic hydrocarbons (PAH), aromatic amines and cisplatin by quantitative immunoassays, immunohistochemistry, immunoaffinity chromatography (IAC), atomic absorbance spectrometry (AAS) and P-32-postlabeling. Studies are being conducted to measure PAH-DNA adducts in blood cell DNA of coke oven workers, aluminum plant workers, subjects ingesting charbroiled beef and Army personnel exposed to oil well fires in Kuwait, using the benzo[a]pyrene-DNA enzyme-linked immunosorbent assay (ELISA) and the 6- fold more sensitive dissociation-enhanced lanthanide fluoroimmunoassay (DELFIA). Aromatic amine-DNA adducts are being concentrated from human lung DNA by IAC and quantified by P-32-postlabeling. The extent of cisplatin-DNA adduct formation in nucleated blood cell DNA of cancer patients (measured by cisplatin-DNA ELISA) has been positively correlated with disease response in ovarian cancer patients receiving platinum chemotherapy. This association is being further investigated in blood samples from previously-untreated ovarian cancer patients, all given the same protocol. Platinum-DNA adducts have been measured in placenta and blood cell DNA from a woman given cisplatin and carboplatin for ovarian cancer during pregnancy. This human exposure was modeled in the patas monkey, and adducts were measurable in both maternal and fetal tissues. Blood cell platinum-DNA adducts have been correlated with dose by both ELISA and AAS in samples from testicular cancer patients during 3 cycles of platinum-drug chemotherapy. An antiserum specific for the anti-AIDS drug, 3'-azido-2',3'-dideoxy-thymidine (AZT), has been used to establish an RIA and immunohistochemical localization for AZT incorporated into the DNA of human HL 60, hamster Chinese hamster ovary (CHO) and mouse NIH 3T3 cell lines. Incorporation of AZT into DNA by radiolabeling correlated well with that determined by RIA. The highest concentrations of AZT incorporated into CHO cell chromosomal DNA colocalized with regions of DNA in Z conformation.