MECHANISMS OF HORMONE AND TRANSMITTER SECRETION

我们的工作继续集中在导致融合的过程中， 颗粒和质膜， 包括嗜铬细胞，胰岛的数据细胞，神经 终末和粘蛋白分泌细胞从受囊性病变影响的组织 纤维化 分泌过程中的接触和融合过程可能是 由钙结合蛋白synexin（膜联蛋白VII）介导。 我们有 了解到这种蛋白质是免疫抑制药物的靶点， 环孢菌素和FK 506，并且它在 神经系统和其他地方。 膜联蛋白脂皮质素I（膜联蛋白I）可 是钡激活分泌的介体， 脂皮质素I活性似乎受到核苷酸（包括cAMP）的调节 和ATP。 已显示Endonexin II（膜联蛋白V）构成了一个重要的 一部分基质囊泡用于产生新骨，并成为 通道，使钙沉积在适当的位置。 对神经递质分泌的进一步研究表明， 分泌可以独立于细胞外钙储存而发生，并且 以比常规分泌慢得多的速率发生。 垂体 促性腺激素细胞也会对钙信号做出反应，但在这种情况下， 由apamin敏感性K+通道运行的膜电位振荡 引起细胞内钙浓度的波动。 MPTP，a 神经毒素，导致帕金森综合征的人，也导致 金鱼也有类似的症状，就像人类的疾病一样， 用MAO-B抑制剂L-丙炔苯丙胺进行防御。 的核苷酸 CFTR的结合折叠部分已被表达，并显示是一种 平面脂质双层中的核苷酸门控阴离子通道。 两个A（1）- 已经发现腺苷拮抗剂可以激活来自 囊性纤维化细胞和DPCPX正在开发用于临床试验。 一种大分子磷脂酶-A抑制剂已被证明能抑制粘蛋白 囊性纤维化和对照细胞的分泌物。

Our work continues to focus on the processes leading to fusion between granule and plasma membranes during exocytotic secretion from cells, including chromaffin cells, data cells from Islets of Langerhans, nerve terminals, and mucin secreting cells from tissues affected by cystic fibrosis. The contact and fusion processes during secretion may be mediated by the calcium binding protein synexin (annexin VII). We have learned that this protein is a target for the immunosuppressant drugs, cyclosporin and FK506, and that it is heterogeneously distributed within the nervous system and elsewhere. The annexin lipocortin I (annexin I) may be the mediator of barium activated secretion, and both synexin and lipocortin I activity appear to be modulated by nucleotides, including cAMP and ATP. Endonexin II (annexin V) has been shown to make up a substantial portion of the matrix vesicles used to create new bone, and to be the pathway for allowing calcium to be laid down at the appropriate site. Further studies on secretion of neurotransmitter have indicated that secretion can occur independently of extracellular calcium stores, and occurs at a much slower rate than conventional secretion. Pituitary gonadotrophs also secrete in response to a calcium signal, but in this case oscillations in membrane potential, run by an apamin-sensitive K+ channel cause oscillations in calcium concentration within the cells. MPTP, a neurotoxin which causes a Parkinsonian syndrome in man, also causes a similar syndrome in the goldfish, which like the human disease can de defended against with the MAO-B inhibitor L-deprenyl. The nucleotide binding fold portion of the CFTR has been expressed and shown to be a nucleotide-gated anion channel in planar lipid bilayers. Two A(1)- adenosine antagonists have been found to activate chloride efflux from cystic fibrosis cells, and DPCPX is being developed for a clinical trial. A macromolecular phospholipase-A inhibitor has been shown to inhibit mucin secretion from cystic fibrosis and control cells.