MECHANISMS OF HORMONE AND TRANSMITTER SECRETION

对人类和小鼠的突触蛋白基因进行了测序，并将其分配给 同源染色体。人类基因位于10q21.1- 21.2，而小鼠基因定位于14号染色体上。 在剪接方面，联蛋白基因被发现是高度同源的 交叉点位置，但与其他组织截然不同 膜联蛋白家族的成员。联欣的半衰期被发现为 约40小时，而蛋白质合成抑制剂阻止分泌 牛嗜铬细胞在相当短的时间内。因此，在 除了联结蛋白外，分泌还需要其他蛋白质 进程。人细胞色素b561，一种主要的跨膜蛋白 嗜铬颗粒，被发现有5个跨膜区 比之前假设的六个要多。膜电位被发现是 调节垂体促性腺激素细胞胞浆钙振荡。 发现M3-M受体能增强葡萄糖诱导的胰岛素 从大鼠胰岛释放。糖尿病诱导剂四氧嘧啶被发现 激活小鼠胰岛β细胞的K(ATP)通道。一部小说， 脑脊液/血管分流术用于异种胰岛的植入 在糖尿病动物身上。对人胰岛进行了分析 首次提出了电生理学方法。获得了证据 1CRAC电流通过效应控制胰岛的电活动 胆碱能受体。几何序列(三半法则) 它准确地描述了允许的多重电导 离子通道的状态。离子通道结构的理论模型 构建了淀粉样β蛋白的融合蛋白。黄嘌呤类药物(CPX)和 一种类似的化合物(DAX)被发现能激活氯从 囊性纤维化细胞。IND被提交给FDA和临床 测试正在计划中。发现摄取了抗坏血酸和脱氢抗坏血酸 通过不同的机制被成纤维细胞吸收。

The human and mouse synexin genes were sequenced, and assigned to homologous chromosomes. The human gene was located at position 10q21.1- 21.2, while the mouse gene was located on chromosome homologous 14. The synexin genes were found to be highly homologous in terms of splice junction location, but quite distinct from the organization of other members of the annexin family. The half life of synexin was found to be ca. 40 h, and inhibitors of protein synthesis blocked secretion from bovine chromaffin cells in an appreciably shorter time. Thus, in addition to synexin other proteins are required for the secretory process. Human cytochrome b 561, a major transmembrane protein of chromaffin granules, was found to have 5 transmembrane domains rather than the six previously hypothsized. membrane potential was found to regulate cytoplasmic calcium oscillations in pituitary gonadoptrophs. M3-muscarinic receptors were found to potentiate glucose-induced insulin release from rat islets. The diabetogenic agent alloxan was found to activate K (ATP) channels in mouse pancreatic beta cells. A novel, cerebrospinal/vascular shunt was developed to implant xenobiotic islets in diabetic animals. Human pancreatic islets were analyzed by electrophysiologic methods for the first time. Evidence was obtained that 1CRAC currents control electrical activity in islets through effects on cholinergic receptors. A geometric sequence (the three-halves rule) was developed which accurately describes allowed multiple conductance states of ion channels. A theoretical model of the ion channel structure of the amyloid beta protein was constructed. A xanthine drug (CPX) and an analog compound (DAX) were found to activate chloride efflux from cystic fibrosis cells. An IND was filed with the FDA and clinical testing is planned. Uptake of ascorbate and dehydroascorbate were found to be taken up by fibroblasts by separate mechanisms.