MECHANISMS OF HORMONE AND TRANSMITTER SECRETION
MECHANISMS OF HORMONE AND TRANSMITTER SECRETION
批准号:
5201921
负责人:
H B POLLARD
金额:
$0.0万
依托单位国家:
美国
项目类别:
财政年份:
--
资助国家:
美国
项目状态:
未结题
起止时间:
至
关键词:
alloxan amyloid proteins annexins ascorbate calcium flux chloride channels chromaffin cells cytochrome b exocytosis genetic mapping glucose metabolism granule hormone regulation /control mechanism human genetic material tag human subject human tissue insulin laboratory mouse laboratory rat membrane potentials membrane transport proteins molecular cloning pancreatic islets potassium channel secretion
中文摘要
对人类和小鼠的联联蛋白基因进行了测序,并进行了分配
英文摘要
The human and mouse synexin genes were sequenced, and assigned to
homologous chromosomes. The human gene was located at position 10q21.1-
21.2, while the mouse gene was located on chromosome homologous 14. The
synexin genes were found to be highly homologous in terms of splice
junction location, but quite distinct from the organization of other
members of the annexin family. The half life of synexin was found to be
ca. 40 h, and inhibitors of protein synthesis blocked secretion from
bovine chromaffin cells in an appreciably shorter time. Thus, in
addition to synexin other proteins are required for the secretory
process. Human cytochrome b 561, a major transmembrane protein of
chromaffin granules, was found to have 5 transmembrane domains rather
than the six previously hypothsized. membrane potential was found to
regulate cytoplasmic calcium oscillations in pituitary gonadoptrophs.
M3-muscarinic receptors were found to potentiate glucose-induced insulin
release from rat islets. The diabetogenic agent alloxan was found to
activate K (ATP) channels in mouse pancreatic beta cells. A novel,
cerebrospinal/vascular shunt was developed to implant xenobiotic islets
in diabetic animals. Human pancreatic islets were analyzed by
electrophysiologic methods for the first time. Evidence was obtained
that 1CRAC currents control electrical activity in islets through effects
on cholinergic receptors. A geometric sequence (the three-halves rule)
was developed which accurately describes allowed multiple conductance
states of ion channels. A theoretical model of the ion channel structure
of the amyloid beta protein was constructed. A xanthine drug (CPX) and
an analog compound (DAX) were found to activate chloride efflux from
cystic fibrosis cells. An IND was filed with the FDA and clinical
testing is planned. Uptake of ascorbate and dehydroascorbate were found
to be taken up by fibroblasts by separate mechanisms.
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MECHANISMS OF HORMONE AND TRANSMITTER SECRETION
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批准号:3875717
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项目类别:
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资助金额:$0.0万
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负责人:H B POLLARD
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依托单位:
MECHANISMS OF HORMONE AND TRANSMITTER SECRETION
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批准号:3776186
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资助金额:$0.0万
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财政年份:--
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负责人:H B POLLARD
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依托单位:
MECHANISMS OF HORMONE AND TRANSMITTER SECRETION
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批准号:3917564
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资助金额:$0.0万
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负责人:H B POLLARD
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依托单位:
MECHANISMS OF HORMONE AND TRANSMITTER SECRETION
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批准号:3964273
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资助金额:$0.0万
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负责人:H B POLLARD
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依托单位:
MECHANISMS OF HORMONE AND TRANSMITTER SECRETION
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批准号:3940462
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资助金额:$0.0万
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财政年份:--
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负责人:H B POLLARD
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依托单位:
MECHANISMS OF HORMONE AND TRANSMITTER SECRETION
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批准号:4689402
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资助金额:$0.0万
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负责人:H B POLLARD
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依托单位:
MECHANISMS OF HORMONE AND TRANSMITTER SECRETION
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批准号:3754075
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资助金额:$0.0万
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负责人:H B POLLARD
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依托单位:
MECHANISMS OF HORMONE AND TRANSMITTER SECRETION
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批准号:3839732
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:H B POLLARD
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依托单位:
MECHANISMS OF HORMONE AND TRANSMITTER SECRETION
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批准号:3854681
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:H B POLLARD
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依托单位:
海外基金