PATHOPHYSIOLOGY OF LUNG LIQUID & PROTEIN EXCHANGE

这项提案的总体目标是：a)审查几个方面 在正常和急性损伤的肺中液体和溶质交换；b) 了解肺泡-呼吸道屏障的肺泡液和 蛋白质清除；c)测量与以下各项有关的间质力学 流体的运动和堆积；d)量化 生理、神经和体液因素对肺血流动力学和肺液的影响 平衡；以及e)确定肺淋巴是否适合于 肺水平衡的实验研究。将在以下地点进行的研究 胎羊、新生羊和成年羊以及狗和兔也包括：1) 中性粒细胞相关性急性肺的病理生理学研究 微血管损伤；2)主动代谢和被动代谢检查 肺泡液清除的静液机制；3)测定 纵隔尾侧的数量解剖、血供和生理 绵羊的淋巴结；4)身体和体液因素的检测 参与胎羊和新生羔羊的肺液和溶质平衡；5) 液体和蛋白质交换的时间进程和位置的确定 在隔离肺中跨越肺泡-呼吸道屏障；6)澄清 局灶性和弥漫性中枢神经系统损伤对肺的作用 体液平衡；7)身体和体液因素的检查 非麻醉成人正常肺和损伤肺的肺液平衡 绵羊；以及8)肺间质力学的测量 与流体平衡的关系。

The general objectives of this proposal are: a) to examine several aspects of fluid and solute exchange in normal and acutely-injured lungs; b) to understand the alveolar-airway barrier in terms of alveolar fluid and protein clearance; c) to measure interstitial mechanics in relation to fluid movement and accumulation; d) to quantify the interactions of physical, neural and humoral factors on lung hemodynamics and lung fluid balance; and e) to establish the appropriateness of lung lymph for the experimental study of lung fluid balance. The studies to be conducted in fetal, newborn and adult sheep and also in dogs and rabbits include: 1) study of the pathophysiology of neutrophil-associated acute lung microvascular injury; 2) examination of active metabolic and passive hydrostatic mechanisms of alveolar fluid clearance; 3) determination of the quantitative anatomy, blood supply and physiology of the caudal mediastinal lymph node in sheep; 4) examination of physical and humoral factors involved in lung fluid and solute balance in the fetal and newborn lamb; 5) determination of the time course and site of liquid and protein exchange across the alveolar-airway barrier in the isolated lung; 6) clarification of the role of focal and diffuse central nervous system injury on lung fluid balance; 7) examination of the physical and humoral factors involved in lung fluid balance in normal and injured lungs in unanesthetized adult sheep; and 8) measurement of the mechanics of the lung's interstitium in relation to fluid balance.