TRANSFORMATION BY HUMAN CMV

人巨细胞病毒（CMV）感染与几种 肿瘤性疾病，包括前列腺癌， 结肠癌、宫颈癌和卡波西肉瘤。 形态 CMV Towne的转化区II（mtrII）已定位于980 含有三个推定的开放阅读框架（ORF）的碱基对片段 79、83和34个氨基酸（aa）。 同源区存在于菌株中 Towne，AD 169和Tanaka。 然而，田中mtrII减少了转换 与Towne和AD 169相比， 一个79 aa（ORF）存在于两个 Towne和AD169在Tanaka中被截短，表明它可能是至关重要的 进行有效的转化。 980 bp mtrII DNA片段的启动子活性已被证实是 研究了 实验表明，980 bp的共线性DNA 当以有义方向插入时， ORF，但不是相反方向。 在进一步的实验中， 不同的启动子区域通过分别测试 左侧440 bp和右侧540 bp片段。 启动子活性 在所有三种菌株中显示出相似性，这意味着这不是一种 在转型的因素，从而强调79 aa的重要性 ORF。

Human cytomegalovirus (CMV) infection has been associated with several neoplastic diseases, including prostatic carcinoma, adenocarcinoma of the colon, cervical carcinoma, and Kaposi's sarcoma. The morphological transforming region II (mtrII) of CMV Towne has been localized to a 980 base-pair fragment containing three putative open reading frames (ORFs) of 79, 83, and 34 amino acids (aa). Homologous regions exist in strains Towne, AD169 and Tanaka. However, Tanaka mtrII has reduced transforming activity when compared to Towne and AD169. A 79 aa (ORF) present in both Towne and AD169 is truncated in Tanaka, indicating that it may be crucial for efficient transformation. The promoter activity of the 980 bp mtrII DNA fragment has been investigated. The experiments showed that the 980 bp colinear DNA acts as a promoter when inserted in the sense orientation with reference to the ORF but not in the opposite orientation. In further experiments, two distinct promoter regions were detected by separately testing the left-hand 440 bp and the right-hand 540 bp fragment. Promoter activity was shown to be similar in all three strains, implying that this is not a factor in transformation, thus emphasizing the importance of the 79 aa ORF.