GENETIC ANALYSIS OF HUMAN CELLULAR GENES IN NEOPLASTIC TRANSFORMATION

人类基因图谱项目包括校内和校外 与科学界的几个团体合作。 的 遗传科已开发了有效的设施， 通过不同的方式和方法来维持这一方案。 这些包括（1）混合电池板的创建和维护， 在人和啮齿动物来源的体细胞杂交后， 在特殊培养基中进行杂种选择;（2）生化和 细胞系的核学特征;（3）原位杂交 人类中期染色体的分子克隆;（4）检测 通过筛选人DNA的限制性片段长度多态性;和 (5)使用多态性人类基因座和将基因置于 多态性研究中心的染色体图谱 Humain（CEPH）协作。 其他技术还包括使用 聚合酶链反应扩增和电泳分析 RNA或蛋白质结合产物。 这些方法总共有 使我们能够绘制许多不同的产物，如原癌基因， 生长因子，肿瘤相关产品，细胞受体， 免疫球蛋白超家族和趋化物质。 首席研究员在人类基因组计划中担任 当选为人类基因组组织成员， 国际比较基因图谱委员会 地位 正在进行的合作项目以表格形式列于本项目 本报告的描述。

The human Gene Mapping project involves intramural and extramural collaborations with several groups of the scientific community. The Genetics Section has developed efficient facilities for establishing and maintaining this program through different approaches and methodologies. These include (1) the creation and maintenance of a hybrid cell panel, following hybridization of somatic cells of human and rodent origins and hybrid selection in special culture media; (2) biochemical and karyological characterizations of cell lines; (3) in situ hybridization of molecular clones to human metaphase chromosomes; (4) detection of restriction fragment length polymorphisms by screening human DNAs; and (5) linkage estimation using polymorphic human loci and placing genes on chromosome maps in the context of the Centre D'Etude du Polymorphisme Humain (CEPH) collaboration. Other techniques have also included the use of polymerase chain reaction amplification and electrophoretic analysis of RNA or protein binding products. Altogether these approaches have enabled us to map a number of disparate products such as proto-oncogenes, growth factors, tumor-related products, cell receptors, members of the immunoglobulin superfamily, and chemotactic substances. The principal investigator serves in the human genome projects as an elected member of the Human Genome Organization as well as chairman of the International Committee on Comparative Gene Mapping. Status of ongoing collaborative projects are listed in tabular form in the Project Description of this report.