GENE AND TRANSGENE REGULATION IN THE DEVELOPING MOUSE

哺乳动物的基因调节和控制正在许多方面进行研究 研究项目，其中许多反映了与其他国家的合作 实验室。 肿瘤发生和分化的研究。1.一个处于休眠状态的癌基因 在转基因小鼠的眼睛晶状体中被激活 编码细菌DNA重组酶的转基因。转基因在中国的激活 活体将被用来研究癌症的分子遗传学 在哺乳动物有机体中的启动。2.癌基因介导的永生化 对原始脑下垂体神经元的研究为理解 人类中枢性性腺功能减退症的分子遗传学。3.对人的分析 子宫癌揭示了导致癌症的不同机制。 P53抑癌基因失活。 人类高谢病的小鼠模型。在一项重要的技术壮举中， 导致这种频繁脂肪的葡萄糖脑苷酶基因突变 储存障碍通过同源基因被引入小鼠生殖系 胚胎干细胞的重组，以及评估新的 治疗的途径可能很快就会到来。 对染色质结构和转基因活性进行了比较 表达鸡珠蛋白基因。该基因的基因座控制区是 足以导致介导基因的染色质构型 表情。 磷酸酪氨酸磷酸酶在哺乳动物发育中的作用 借助新克隆的小鼠基因进行编码 非受体类型酶。这种酶在整个发育过程中都有表达。 小鼠T细胞受体Zeta链的功能分析。 在Zeta链基因中通过 小鼠胚胎干细胞的同源重组。之后 在小鼠生殖细胞系中引入，这些突变有望 阐明Zeta链蛋白在胸腺细胞成熟和分化中的作用 它在T细胞受体的组装和表达中的要求。

Mammalian gene regulation and control is being studied in a number of research projects, many of them reflecting collaborations with other laboratories. Studies on oncogenesis and differentiation. 1. A dormant oncogene has been activated in the eye lens of transgenic mice with the help of a second transgene encoding a bacterial DNA recombinase. Transgene activation in vivo will be utilized to investigate the molecular genetics of cancer initiation in the mammalian organism. 2. Oncogene mediated immortalization of primordial pituitary neurons has opened a way to understand the molecular genetics of central hypogonadism in man. 3. Analysis of human uterine cancers has revealed different mechanisms leading to the inactivation of the p53 tumor suppressor gene. Mouse models of human Gaucher disease. In an important technical feat, mutations of the glucocerebrosidase gene that cause this frequent lipid storage disorder have been introduced in the mouse germ line via homologous recombination in embryonic stem cells, and a mouse model to evaluate new avenues of therapy may be at hand soon. Chromatin structure and transgene activity have been compared in mice that express a chicken globin gene. The locus control region of this gene is sufficient to cause a chromatin configuration that mediates gene expression. The role of phosphotyrosine phosphatases in mammalian development is being addressed with the help of newly cloned mouse gene that encodes a non-receptor type enzyme. The enzyme is expressed throughout development. Functional analysis of the Zeta chain of the T-cell receptor in the mouse. Specific mutations have been introduced in the Zeta chain gene via homologous recombination in murine embryonic stem cells. After introduction in the mouse germ line, these mutations are expected to elucidate the role of the Zeta chain protein in thymocyte maturation and its requirement in the assembly and expression of the T-cell receptor.