SPECIALIZED CENTER OF RESEARCH IN ARTERIOSCLEROSIS

这是一个紧密结合的项目，深入调查 脂蛋白相互作用以及与不同细胞和 身体组织，以及脂蛋白是如何产生和获得的 进入并积聚在动脉壁，在那里影响细胞 新陈代谢。个体恒河猴是我们许多人关注的焦点 学习。这些主要关注的问题将在几个层面上得到解决-- 单个脂蛋白和脂蛋白的结构及其相互作用 亚类；其组成的载脂蛋白的异质性；体内 以及载脂蛋白和脂蛋白的体外形成和代谢 肝、肠和血浆室内；控制 新生极低密度脂蛋白的组装和细胞内转运的因素 血浆低密度脂蛋白的前体；家族性和营养决定因素 体内脂蛋白图谱强调不同的反应 个别动物要向高脂肪发起挑战，特别要注意 这些的分子基础；相互作用的特异性决定因素 脂蛋白及其亚类与动脉壁细胞的关系 脂蛋白及脂蛋白亚类对肝细胞生长的影响 平滑肌细胞和巨噬细胞在组织培养中的作用 影响脂蛋白水平的血流动力学因素 培养中的动脉壁和细胞；脂蛋白大小的决定因素 在正常和高脂血症动物中。主要强调的是 主要类型的脂蛋白和载脂蛋白的异质性， 无论这些是由遗传、营养和生物合成控制的，还是 其他代谢决定因素。

This is a closely integrated program which intensively investigates lipoprotein interactions with one another and with various cells and tissues of the body, as well as how lipoproteins are produced and gain access to and accumulate in the artery wall, there to affect the cell metabolism. The individual Rhesus monkey is the focus of many of our studies. These central concerns will be approached at several levels--the structure and the interactions of individual lipoproteins and lipoprotein subclasses; the heterogeneity of their component apoproteins; the in vivo and in vitro formation and metabolism of apoproteins and lipoproteins by the liver, the intestine and within the plasma compartment; the controlling factors in the assembly and intracellular transport of nascent VLDL, the precursor of plasma LDL; the familial and nutritional determinants of lipoprotein profiles in vivo emphasizing the differential response of individual animals to high fat challenge with particular attention to the molecular basis of these; the specificity determinants of the interaction of lipoproteins and lipoprotein subclasses with cells of the artery wall and liver; the effects of lipoproteins and lipoprotein subclasses on the smooth muscle cells and macrophages in tissue culture; the effects of hemodynamic factors in modulating the influence of lipoproteins on the artery wall and on cells in culture; the determinants of lipoprotein size in normal and hyperlipidemic animals. Major emphasis is given to the heterogeneity within the major classes of lipoproteins and apoproteins, whether these are controlled by genetic, nutritional, and biosynthetic or other metabolic determinants.