HYBRID (EBQQ) MASS SPECTROMETER

这项请求是为了让混合质谱计成为质量的一部分 堪萨斯大学光谱分析实验室，为 化学系，药物化学系，药物化学系， 药理学和毒理学，KU医学中心和 生物医学和生物分析研究。主要的需求是增加 快速原子轰击(FAB)能力。这已经是一种既定的 10年来不断增长的能力和样本量的服务实验室。一个 目前共有30个小组使用该实验室，7/12通过 本财年，该实验室已经运行了1690个样本，并将在 七月。这项工作是在三个设施仪器上进行的，一个 VARIAN-NUT CH5，Nermag四极柱GC/MS系统和高分辨率 VG Zab HS.Nermag和Zab用于大部分样品。 CH5(购买于1970年)作为FAB乐器已经过时。Nermag是 目前已通过探针和GC实验达到饱和使用。扎布已经 用常规的精确质量和FAB分析达到接近饱和。 从1989年7月开始，对FAB分析的需求增加了6 前一年的次数。预计FAB的工作将再翻一番 进行了支持四个基团的多肽分子量实验 刚刚开始使用质谱学对多肽进行表征。 多肽项目涉及识别和本地化 小肽中的修饰氨基酸。的MS/MS能力 混合将用于提供中未提供的序列信息 法布实验。单位质量前兆和碎片离子分辨率 在CAD实验中通过杂交获得的将是多肽的关键 涉及溴和氚标记多肽的项目。两组 目前正在合成配位化合物，并已成为主要的 FAB用户确认中间体和最终产品。两者都有 各小组在从他们的研究中获得分子离子方面取得了喜忧参半的成功。 含有金属的样品。经常观察到脱金属物种 在FAB光谱中。类似的化合物已被菲尔德表征 描述(FD)。将FD用于配位化合物提供了一种 软电离方法，避免了基质化学的复杂性。

This request is for a Hybrid Mass Spectrometer to be part of the Mass Spectrometry Laboratory at the University of Kansas, serving the Departments of Chemistry, Medicinal Chemistry, Pharmaceutical Chemistry, Pharmacology and Toxicology, KU Medical Center and the centers for Biomedical and Bioanalytical Research. The primary need is for increased fast atom bombardment (FAB) capability. This has been an established service lab with growing capability and sample load for 10 years. A total of thirty groups currently use the lab and at 7/12 through the fiscal year the lab has run 1690 samples and will easily reach 2500 by July. This work is being carried out on three facility instruments,a Varian-NUT CH5, a Nermag quadrupole GC/MS system, and a high resolution VG ZAB HS. The Nermag and the ZAB are used for the bulk of the samples. The CH5 (bought in 1970) is obsolete as a FAB instrument. The Nermag is currently saturated in use by probe and GC experiments. The ZAB has reached near saturation with routine exact mass and FAB analysis. Starting in July of 1989 the demand for FAB analysis has increased 6 times from the previous year. FAB work is expected to double again as peptide molecular weight experiments are done in support of four groups just beginning to use mass spectrometry for peptide characterization. The peptide projects involve the identification and localization of modified amino acids in small peptides. The MS/MS capability of the hybrid will be used to provide the sequence information not available in the FAB experiments. The unit mass precursor and fragment ion resolution obtainable with a hybrid in CAD experiments will be vital for peptide projects involving bromine and deuterium labeled peptides. Two groups are currently synthesizing coordination compounds and have been the major FAB users for confirmation of intermediates and final products. Both groups have had mixed success in obtaining molecular ions from their metal containing samples. Frequently demetallated species are observed in the FAB spectra. Similar compounds have been characterized by Field Desroption (FD). The use of FD for coordination compounds provides a soft ionization method that avoids the complications of matrix chemistry.