INTERACTIVE THREE-DIMENSIONAL GRAPHICS COMPUTER SYSTEM

我们建议增加一项计算机化三维图像处理设施 北京国际机场细胞测量设施的改造与分析 UCI发育生物学中心(DBC)。细胞计量学设备 已经提供了激光扫描共聚焦显微镜技术， 流式细胞术、图像分析和处理以及单细胞 为DBC成员以及一些外部用户提供微量注射。3D 成像设备将主要使用由生成的堆叠2D图像 荧光标记的全组织样品和遗嘱的共聚焦显微镜 使3D重建、旋转、伪彩色和体积成为可能 在几分钟内完成测量，而不是耗费数小时 利用目前的技术。该系统还将被用来操纵 通过磁共振成像显微镜获得的图像集。 该设备将最初用于研究基因的表达 果蝇胚胎和成像盘中的细胞黏附分子， 海胆胚胎和海胆胚胎细胞谱系模式分析 脊椎动物神经系统发育、图谱和实验 下丘脑发育神经元生长锥的研究 脊椎动物视觉系统，研究发育的细胞方面 在躯体感觉皮层，负责摄食的神经细胞 九头蛇的反应，以及已识别神经元的电学性质 在果蝇身上。

We propose to add a facility for computerized three-dimensional image reconstruction and analysis to the Cytometric Facility at the Developmental Biology Center (DBC), UCI. The Cytometric Facility already provides the techniques of laser scanning confocal microscopy, flow cytometry, image analysis and manipulation, and single-cell microinjection for DBC members as well as some outside users. The 3D imaging facility will work primarily from stacked 2D images generated by confocal microscopy of fluorescent-labeled whole tissue samples and will make possible 3D reconstruction, rotation, pseudocoloring and volume measurements in a matter of minutes rather than the many hours consumed using present technology. The system will also be used to manipulate image sets obtained by magnetic resonance imaging microscopy. The equipment will be used initially in studies of the expression of cell adhesion molecules in Drosophila embryos and imaginal discs, analysis of cell lineage patterns in sea urchin embryos and in the developing vertebrate nervous system, mapping and experimental investigation of developing neuronal growth cones in the lower vertebrate visual system, investigating cellular aspects of development in the somatosensory cortex, the nerve cells responsible for the feeding response of hydra, and the electrical properties of identified neurons in Drosophila.