400 MHZ WIDE BORE NMR SPECTROMETER

需要资金购买一台400兆赫大口径核磁共振光谱仪 支持高分辨率结构研究、细胞内代谢 使用灌流器官和细胞培养系统的研究， 大分子动力学的表征，以及体内成像和 啮齿动物肿瘤模型的光谱分析。所请求的仪器是 通用电气400 MHz多核核磁共振谱仪 可互换垫片和渐变集，既允许高分辨率 液体起作用和显微成像。该仪器将配置有 允许质子、氟、磷、碳、氮、 氯、氢、钠和其他原子核，温度可变 功能，阵列处理器，允许快速处理图像和 2D数据集和宽带解耦器，以允许间接检测 X核。此外，我们还将为特殊情况构建探针 四极核的应用和大体积的应用 这是必要的。本文书将服务的调查人员包括 生物物理学、药理学和生物化学的教授。此外 为了缓解IBM-270 MHz仪器目前的过载问题 新的化合物结构表征医学中心 仪器将支持新陈代谢的代谢表征工作 含氟化合物，抗肿瘤药物，和表征 对几种治疗策略的代谢和解剖反应。在……里面 此外，该仪器还将支持阴离子的机理研究 膜系统中的传输，微成像技术的发展， 以及大分子动力学的表征。共享仪器 将被安置在一个电流共享的活体核磁共振光谱仪旁边 生物物理系的设施。

Funds are requested for a 400 MHz wide-bore NMR spectrometer that will support high resolution structural studies, intracellular metabolic studies using perfused organs and cell culture systems, the characterization of macromolecular dynamics, and in vivo imaging and spectroscopy on rodent tumor models. The instrument requested is a General Electric 400 MHz multinuclear NMR spectrometer operating with an interchangeable shim and gradient set that permits both high resolution liquids work and micro-imaging. The instrument will be configured with probes to permit proton, fluorine, phosphorous, carbon, nitrogen, chlorine, deuterium, sodium, and other nuclei, variable temperature capabilities, an array processor to permit rapid handling of image and 2D data sets, and a broad band decoupler to permit indirect detection of X nuclei. In addition, we will construct probes for special applications to quadrupole nuclei and large volume applications as necessary. The investigators to be served by this instrument include professors in biophysics, pharmacology, and biochemistry. In addition to relieving the current excessive load on the IBM-270 MHz instrument in the Medical Center for structural characterization of compounds, the new instrument will support work on metabolic characterization of metabolism of fluorine compounds, of anti-tumor drugs, and characterization of metabolic and anatomic response to several therapeutic strategies. In addition the instrument will support mechanistic studies of anion transport in membrane systems, development of micro-imaging techniques, and characterization of macromolecular dynamics. The shared instrument will be housed adjacent to a current shared in vivo NMR spectrometer facility in the Biophysics Department.