PURCHASE OF A HIGH FIELD NMR SPECTROMETER

需要在此拨款建议中要求的400 MHz NMR光谱仪 伦斯勒理工学院化学系 支持几个NIH资助的研究项目。 核心用户群 需要一个高场核磁共振仪器与复杂的软件， 研究大型生物化学品，如蛋白质，多肽和相关 物种以及结构复杂的“小”有机分子。 具体应用包括1）蛋白质（内皮素， 丙甲霉素、短杆菌肽和溶菌酶的离子结合位点），2）药物 与模型寡核苷酸的相互作用，3）自组装无机 双螺旋，执行电荷转移功能， 类似于光合系统，和4）离子通道。 其他 正在调查的生物学相关项目包括研究 聚醚抗生素，胆固醇生物合成抑制剂，鸦片制剂， 显示抗AIDS活性的C-核苷和糖苷 抗生素 有机合成中的不对称方法 正在开发具有生物和/或医药活性的分子。 高场核磁共振光谱将发挥关键作用， 了解立体控制机制。 合成复杂大环化合物的新方法 系统正在开发中;这些大环将结合金属离子 和小的有机分子，并将使立体选择性反应， 进行 高场核磁共振也将是非常宝贵的发展新的 阿片受体的光亲和标记。

The 400 MHz NMR spectrometer requested in this grant proposal is needed by the Chemistry Department at Rensselaer Polytechnic Institute to support several NIH-funded research programs. The core user group requires a high-field NMR instrument with sophisticated software to study large biochemicals such as proteins, polypeptides and related species as well as structurally complex "small" organic molecules. Specific applications include the study of 1) proteins (endothelin, alamethicin, gramicidin and ion-binding sites of lysozyme), 2) drug interactions with model oligonucleotides, 3) self-assembled inorganic double helicates that perform charge transfer functions somewhat analogous to photosynthetic systems, and 4) ion channels. Other biologically relevant projects under investigation include the study of polyether antibiotics, inhibitors of cholesterol biosynthesis, opiates, C-nucleosides that exhibit anti-AIDS activity, and glycoside antibiotics. Enantioselective methods for synthesis of organic molecules with biological and/or medicinal activity are being developed. High field NMR spectroscopy will play a crucial role in the understanding of stereocontrol mechanisms. New synthetic methods for the construction of complex macrocyclic systems are under development; these macrocycles will bind metal ions and small organic molecules and will enable stereoselective reactions to be carried out A high field NMR also will be invaluable for the development of new photoaffinity labels for the opiate receptors.