PATHOPHYSIOLOGY OF LUNG LIQUID AND PROTEIN EXCHANGE

本建议的总体目标是：a）审查几个方面 正常和急性损伤肺中的液体和溶质交换; B） 从肺泡液的角度了解肺泡-气道屏障， 蛋白质清除率; c）测量与以下相关的间质力学： 流体运动和积聚; d）量化 物理、神经和体液因素对肺血流动力学和肺液的影响 平衡;和e）建立肺淋巴液的适当性， 肺液体平衡的实验研究 研究将在 胎儿、新生儿和成年绵羊以及犬和兔包括：1） 嗜水气单胞菌相关性急性肺损伤的病理生理学研究 微血管损伤; 2）主动代谢和被动代谢检查 肺泡液体清除的流体静力学机制; 3）确定 尾侧纵隔的定量解剖、血供和生理 绵羊淋巴结; 4）物理和体液因素检查 参与胎儿和新生羔羊的肺液体和溶质平衡; 5） 确定液体和蛋白质交换的时间进程和位置 在离体肺中穿过肺泡-气道屏障; 6）澄清 局灶性和弥漫性中枢神经系统损伤对肺的作用 体液平衡; 7）检查涉及的物理和体液因素 未麻醉成人正常肺和损伤肺的肺液体平衡 8）测定了绵羊肺组织的机械强度， 与体液平衡有关。

The general objectives of this proposal are: a) to examine several aspects of fluid and solute exchange in normal and acutely-injured lungs; b) to understand the alveolar-airway barrier in terms of alveolar fluid and protein clearance; c) to measure interstitial mechanics in relation to fluid movement and accumulation; d) to quantify the interactions of physical, neural and humoral factors on lung hemodynamics and lung fluid balance; and e) to establish the appropriateness of lung lymph for the experimental study of lung fluid balance. The studies to be conducted in fetal, newborn and adult sheep and also in dogs and rabbits include: 1) study of the pathophysiology of neutrophil-associated acute lung microvascular injury; 2) examination of active metabolic and passive hydrostatic mechanisms of alveolar fluid clearance; 3) determination of the quantitative anatomy, blood supply and physiology of the caudal mediastinal lymph node in sheep; 4) examination of physical and humoral factors involved in lung fluid and solute balance in the fetal and newborn lamb; 5) determination of the time course and site of liquid and protein exchange across the alveolar-airway barrier in the isolated lung; 6) clarification of the role of focal and diffuse central nervous system injury on lung fluid balance; 7) examination of the physical and humoral factors involved in lung fluid balance in normal and injured lungs in unanesthetized adult sheep; and 8) measurement of the mechanics of the lung's interstitium in relation to fluid balance.