BIOMEDICAL RESEARCH SUPPORT

我们正在申请资金购买ACAS 570交互式激光器 由Meridian Instruments of奥克莫斯，MI标记的细胞计数器。 这 仪器是一个集成的荧光工作站， 培养物中粘附细胞的定量荧光分析， 交互式应用，如荧光光漂白，以测量 细胞上或细胞内以及细胞间的分子扩散，激光 用于分选和选择锚定细胞的消融，以及用于 选择性去除细胞器或细胞突起。 介绍的项目 在这项共享仪器补助金中，将采用各种应用程序 ACAS蛋白质在细胞表面的移动性、细胞间通讯 研究，细胞内Ca 2+和pH测量锚定依赖 细胞以及免疫荧光染色，以获得空间和 各种细胞过程的时间分辨率和 神经元、星形胶质细胞、肿瘤细胞和癌基因中的特异性抗原， 转化细胞 与流式细胞荧光法相比， 荧光激活细胞分选（FACS），ACAS系统提供三个 明显的优势，这是必要的研究建议：1） 能够分析和分选锚定细胞，而无需剥离它们 从它们的基质或连接细胞（并可能导致 对细胞结构和功能造成不可逆和不可估量的损害）; 2） 标记物在一个较长的时间段;和3）相互作用的能力 对于所研究的细胞（例如，荧光光漂白 应用）。 因此，与其作为更传统的 ACAS 570交互式激光细胞分析仪是 他们

We are requesting funds to purchase the ACAS 570 Interactive Laser Cytometer which is marked by Meridian Instruments of Okemos, MI. This instrument is an integrated fluorescence workstation which is designed for quantitative fluorescence analysis of adherent cells in culture and for interactive applications, such as fluorescence photobleaching to measure diffusion of molecules on or within cells as well s between cells, laser ablation for sorting and selection of anchored cells, and cell surgery for selective removal of organelles or cell processes. The projects described in this Shared Instrumentation Grant will employ various applications of the ACAS protein mobility of cell surfaces, cell-cell communication studies, intracellular Ca2+ and pH measurements of anchorage-dependent cells as well as immunofluorescence staining to obtain both spatial and temporal resolution of various cellular processes and the distribution of specific antigens in neurons, astrocytes, tumor cells, and oncogene- transformed cells. In comparison to flow cytofluorimetric methods and fluorescence-activated cell sorting (FACS), the ACAS system offers three distinct advantages which are necessary for the studies proposed: 1) the ability to analyze and sort anchored cells without having to strip them from their substratum or connecting cells (and possibly causing irreversible and inestimable damage to cellular structure and function); 2) the markers over a prolonged period of time; and 3) the ability to interact with the cells under study (eg., the fluorescence photobleaching applications). Thus, rather than an alternative to the more conventional instruments, the ACAS 570 Interactive Laser Cytometer is complementary to them.