STUDIES ON HUMAN GLIOMA AND RAT PROSTATE ADENOCARCINOMA CELLS IN VITRO

癌细胞表面的各种产物脱落 可能在粘连丧失、转移、 免疫阻断因子和其他病理生理因素的产生 癌症方面(Black PH，NEJM.303：1315。1980)。人脑胶质瘤 组织培养中的细胞在培养液中产生和脱落的数量要大得多 糖胺多聚糖(GAG)含量高于正常神经胶质细胞 (Glimelius et al Biochem J.172：443,1978)。糖胺多聚糖是 多阴离子化合物，通常与蛋白质核心共价结合。 它们是细胞表面的重要组成部分，是 牵涉到细胞与细胞的相互作用。也有证据表明 对透明质酸酶敏感的保护膜的存在 包括神经胶质瘤在内的肿瘤细胞系，有研究表明 保护性的恶作剧外套可能会阻碍免疫反应和 化疗的疗效。我们的目标是改变或防止 人脑胶质瘤细胞中GAG的合成和释放 文化，并可能使他们更容易受到 化疗和更多的免疫反应。为此目的， 我们单独或联合使用差异剂。 二甲基亚砜(DMSO)、丁酸钠、维甲酸和 地塞米松。已知这些试剂会影响合成 在其他系统中出现堵塞。在细胞上也进行了类似的研究 PA III系来源于大鼠可移植性腺癌 趴着的腺体。该细胞系可以复制原始肿瘤 当接种到Loboud Wistar大鼠并制作成 如果有必要的话，扩大我们的活体研究是可行的。

Shedding of various products from the cell surface of cancer cells may have an important role in loss of adhesion, metastasis, generation of immune-blocking factors and other pathophysiologic aspects of cancer (Black PH, NEJM. 303:1315. 1980). Human glioma cells in tissue culture produce and shed in the media much greater amounts of glycosaminoglycans (GAGs) than normal glial cells (Glimelius et al Biochem J. 172:443, 1978). Glycosaminoglycans are polyanionic compounds, usually bound covalently to a protein core. They are a prominent component of the cell surface and are implicated in cell-cell interaction. There is also evidence for existence of hyaluronidase-sensitive protective coats on some neoplastic cell lines including gliomas, and it has been suggested that the protective GAG coat may impede the immune response an the efficacy of chemotherapy. Our objective was to modify or prevent the synthesis and shedding of GAGs in human glioma cells in culture, and possibly to render them more susceptible to chemotherapy and more immunologically responsive. For this purpose we use, singly or in combination, the differentiating agents dimethyl sulfoxide (DMSO), sodium butyrate, retinoic acid, and dexamethasone. These agents are known to affect the synthesis of GAGs in other systems. Similar studies were also conducted on cell line PA III derived from a transplantable adenocarcinoma of the rat prostrate gland. The cell line can reproduce the original tumor type when innoculated into Loboud Wistar rats and makes it feasible, if necessary, to extend our studies in vivo.