PURCHASE OF HIGH FIELD NMR SPECTROMETER

这项拨款提案要求为竖井提供部分资金， 高分辨率在体高场核磁共振波谱仪 生物分子、模型化合物和聚合物的光谱学 组织和小型哺乳动物。该仪器将由 经过验证的最高磁场磁体，均匀的磁场直径 至少10 mm，以及具有以下所有必要功能的控制台 多维和多量子等先进技术 实验，异核的质子探测，自旋锁定，以及 能够产生复杂脉冲相位的脉冲序列。 光谱仪将由八个核心小组使用 调查员(主要用户)被组织成三个研究领域， 就像几个次级用户一样。该中心的个别项目 调查人员涵盖了广泛的基础研究 分子生物学和生物化学。每个调查员都有一个 建立了利用LESER光谱仪进行核磁共振研究的方案 功能，并且计划书记录了以下附加知识 将通过拟议文书的新能力而获得。 拟利用光谱仪进行研究的领域 包括生物分子结构和动力学，在体内 生物化学、模型化合物结构及其相互关系 分子构象和生物功能之间的关系。特定的 主题包括(部分)血红素蛋白质的结构和动力学， 组蛋白结构、肾脏生理、艾滋病有效药物 细胞表面多糖的处理和构象，如 以及其他几项分子生物学和 生物化学。 拟议的仪器将由UCD核磁共振赞助 设施。该设施已有十多年的既定记录 使调查人员能够实施最先进的核磁共振 技术，以及光谱仪的维护和改造。

This grant proposal requests partial funding for a vertical bore, high field NMR spectrometer for high resolution and in-vivo spectroscopy of biomolicules, model compounds, and peerfused tissues and small mammals. The instrument will consist of the highest proven field magnet, homogeneous magnetic field diameter of at least 10mm, and a console with all features necessary for advanced techniques such as multidimensional and multiple quantum experiments, proton detection of heteronuclei, spin locking, and capagility of generating pulse sequences of complex pulse phase. The spectrometer will be utilized by a core group of eight investigators (Primary Users) organized into three research areas, as will as several secondary users. The individual projects of the investigators cover a wide range of fundamental research in molecular biology and biochemistry. Every investigator has an established program of NMR research with spectrometers of lesser capability and the proposal documents the additional knowledge to be gained by the new capabilities of the proposed instrument. Areas of research to be studied utilizing the proposed spectrometer include structure and dynamics of biomolecules, in-vivo biochemistry, model compound structure, and the relationship between molecular conformation and biological function. Specific topics include (in part) heme protein structure and dynamics, histone structure, renal physiology, drugs effective in AIDS treatment, and conformation of cell surface polysaccharides, as well as several other fundamental studies in molecular biology and biochemistry. The proposed instrument will come under the auspices of the UCD NMR Facility. The Facility has an established record over ten yeears in enabling investigators to implement state of the art NMR techniques, and in spectrometer maintenance and modification.