APPLIED BIOSYSTEMS PROTEIN SEQUENCER & PTH ANALYZER

此共享工具赠款申请是供基金购买的 EPPLEY实验室核心实验室的多肽测序仪 美国癌症及相关疾病研究所 内布拉斯加大学医学中心校区。这个核心实验室 已经包含了一个寡核苷酸合成器和一个肽 合成器，它包括一名全职技术人员。加法 将填补这项研究中的一个重大空白 UNMC的能力；所有蛋白质现在必须转发到 华盛顿大学或威斯康星大学 测序。我们已经确定了 应用程序以及将监督 这个设施的日常运作。多肽测序 能力将扩大到赔偿委员会的所有调查员，以及 事实上，对该地区的所有研究人员，例如林肯，收取- 服务基础。

This Shared Instrument Grant Application is for funds to purchase a peptide sequencer for a core laboratory operated by the Eppley Institute for Research in Cancer and Allied Diseases for the University of Nebraska medical Center campus. This core laboratory already contains an oligonucleotide synthesizer and a peptide synthesizer, and it includes a full-time technician. The addition of the peptide sequencer would fill a major void in the research capabilities for the UNMC; all proteins must now be forwarded to Washington University or the University of Wisconsin for sequencing. We have identified the major user group in the application as well as the advisory group that will oversee the daily operation of this facility. The peptide sequencing capability will be extended to all investigators of the UNMC, and indeed to all researchers in the area, e.g., Lincoln, on a fee-for- service basis.