GENETIC AND MOLECULAR ORGANIZATION OF THE MHC IN THE DOMESTIC CAT

家猫的主要组织相容性复合体（MHC） 使用血清学和分子程序进行表征。 在不相关的猫之间交换皮肤移植物; 75%的皮肤移植是在父母无关的兄弟姐妹之间进行的 而100%的非亲缘猫之间的关系是急性的， 14天内被拒绝。 细胞毒性同种抗血清 来自14个不同的个体， 分析不相关的野猫以确定重叠 免疫遗传特异性 此外，还进行了 美国国立卫生研究院猫群中的九个家庭导致了对 在家系中相互分离的异源单倍型 实验 这些数据被用来推导出第一个猫的MHC （称为弗拉，猫白细胞抗原）检测图表 单倍型。 使用细胞毒性的免疫沉淀实验 分型同种抗血清鉴定I类和II类类型 分子。 用异源DNA探针对猫DNA进行分子分析 人类或小鼠分子探针（I类和II类）显示， 猫的单倍体基因组包含大约20个I类基因座， 和2个II类基因。 家猫I类基因表达 限制性片段长度多态性（RFLP）; 比观察到的RFLP程度低大约五倍 在小鼠，大鼠或猪中，几乎相当于MHC的程度， 在人类和MHC中检测到的基因RFLP- 单形叙利亚仓鼠 I类和II类基因都是 基因定位到猫的染色体B2使用面板 啮齿动物和猫的体细胞杂种。 一个部分cDNA I类基因 从猫T细胞淋巴瘤的cDNA文库中分离的pFLA 2 细胞系目前正在分析中。

The major histocompatibility complex (MHC) in the domestic cat was characterized using serological and molecular procedures. Reciprocal skin grafts were exchanged between unrelated cats; 75% of the skin grafts between the siblings of unrelated parents and 100% of those between the unrelated cats were acutely rejected within 14 days. Cytotoxic alloantisera were derived from 14 different individuals and were used in a population cluster analysis of unrelated feral cats to define overlapping immunogenetic specificities. In addition, pedigree analysis of the nine families in the NIH cat colony led to the description of allogeneic haplotypes which segregated from each other in family experiments. The data were used to derive the first feline MHC (termed FLA, feline leukocyte antigen) chart of detected haplotypes. Immunoprecipitation experiments using cytotoxic typing alloantisera identified both class I and class II type molecules. A molecular analysis of feline DNA using heterologous human or mouse molecular probes (class I and class II) revealed that the cat haploid genome contains approximately 20 class I loci and 2 class II genes. Class I genes of the domestic cat expressed limited restriction fragment length polymorphism (RFLP); approximately five times lower than the extent of RFLP observed in mice, rats, or pigs, and almost equivalent to the extent of MHC gene RFLP that is detected in humans and in the MHC- monomorphic Syrian hamster. Class I and class II genes were both genetically mapped to feline chromosome B2 using a panel of rodent x cat somatic cell hybrids. A partial cDNA class I gene (pFLA2) isolated from a cDNA library of a cat T-cell lymphoma cell line is presently under analysis.