STUDIES OF PHOTOTHERAPY FOR THORACIC MALIGNANCIES

我们的实验室继续进行光动力疗法的研究。 (PDT)敏化治疗胸部恶性肿瘤 用双血卟啉乙醚(DHE)处理恶性细胞 用630纳米的灯光照明。自1987年10月以来，我们已经 研究了光散射介质，脂内，在 不同浓度对提高PDT的疗效有一定的作用。我们有 发现低浓度的体内脂质将提供更大的 通过PDT的细胞毒性，具有更大的光散射效应，尽管 在一段时间内细胞中的敏化剂的损失 与脂质内的孵化。使用幻影有机玻璃模型 对于胸腔，我们已经定义了什么是最佳光线 该模型中的分散浓度是按脂内浓度的顺序进行的 为所有曲面提供相同的照明。我们已经量化了 使用光电二极管检测系统的光散射，构造 由生物工程部负责。我们现在正在调查不同的 不同胸腔肿瘤细胞体外PDT敏感性的研究 包括一种小细胞肺癌变种，一种大细胞 肺癌、间皮瘤、正常肺成纤维细胞和 一种腺癌。对于我们正在定义的每一种细胞系 细胞的荧光特性、细胞大小、细胞 蛋白质，以及对低能量光疗的固有敏感性。这个 低密度脂蛋白受体在细胞水平递送DHE中的作用 也在接受调查。在准备接合的时候 抗致敏剂单抗的体内外研究 在转化成纤维细胞系的光动力疗法中，已进行了45342次 在体外和体内均表现出对光动力疗法的敏感性。 我们已经用PDT治疗了9例支气管腔内疾病患者 并制作了一部演示光疗技术的电影。 两名复发性卵巢癌患者已经接受了 经腹腔内PDT治疗。

Our laboratory has continued investigations of photodynamic therapy (PDT) for the treatment of thoracic malignancies by sensitization of malignant cells with dihematoporphyrin ether (DHE) followed by illumination with 630 nM light. Since October of 1987 we have investigated the use of light scattering media, intra-lipid, in different concentrations to improve the efficacy of PDT. We have found that low concentrations of intra-lipid will provide greater cytotoxicity via PDT, with greater light-scattering effects despite a loss of the sensitizer from the cells during the period of incubation with the intra-lipid. Using a phantom plexiglass model of the thoracic cavity, we have defined what the optimal light scattering concentration in this model is for intra-lipid in order to provide equal illumination to all surfaces. We have quantitated light scattering using a photodiode detection system, constructed by the Bioengineering Division. We are now investigating different in vitro PDT sensitivities of various thoracic, oncologic cell lines including a small-cell lung cancer variant, a large-cell carcinoma of the lung, mesothelioma, normal lung fibroblasts and an adenocarcinoma. For each of these cell lines we are defining fluourescence characteristics of the cells, cell size, cell protein, and inherent sensitivity to low energy phototherapy. The role of LDL receptors in the delivery of DHE at a cellular level is also being investigated. In preparation for conjugation of a monoclonal antibody to the sensitizor, in vitro and in vivo studies of PDT of a transformed fibroblast line, 45342 have been performed showing sensitivity of this line to PDT both in vitro and in vivo. We have now treated 9 patients with endobronchial disease with PDT and have made a movie demonstrating the technique of phototherapy. Two patients with recurrent ovarian carcinomatosis have been treated with intraabdominal PDT.