NEUROREGULATORY ASPECTS OF NEUROMEDIN B

该项目在FY-88开始后停止。二 发表了关于这个主题的文章：J.Neurosci。冰毒。23：161- 172(1988)和Ann.纽约州阿卡德科学，在出版社(1988)。其他 瑞典和意大利的合作者正在准备手稿 神经介素B的免疫组织化学分布 在哺乳动物和尾目动物大脑的特定区域使用抗血清 由本组提供的神经美丁素B。使用这种技术，我们 我们找不到一个广泛的神经介素B特异性网络 大鼠脑内的神经纤维或胞体。这些数据与 含有蛙皮素的神经纤维网络的广泛分布： 蛙皮素和神经梅毒素B属于同一多肽家族， 包含70%的序列相似性，但源自独立的 基因。这种免疫组织化学证据的缺乏可能与 抗血清的特异性或相对较低 组织切片中的神经梅第B浓度，或两者兼而有之。 对其在脑下垂体的定位的进一步研究仍在继续。 北卡罗来纳大学。

This project was discontinued after the beginning of FY-88. Two articles were published on this topic: J. Neurosci. Meth. 23:161- 172 (1988) and Ann. N.Y. Acad. Sci., in press (1988). Other manuscripts are being prepared by collaborators in Sweden and Italy dealing with the immunohistochemical distribution of neuromedin B in selected regions of mammalian and urodele brains using antisera to neuromedin B supplied by this Group. Using this technique, we were unable to find an extensive neuromedin B-specific network of nerve fibers or cell bodies in rat brain. These data contrast to the broad distribution of bombesin-containing nerve fiber network: bombesin and neuromedin B belong to the same peptide family and contain 70% sequence similarity, but are derived from separate genes. This lack of immunohistochemical evidence may be related to the specificity of the antisera or the relatively low concentration of neuromedin B in the tissue slices, or both. Further studies on its localization in the pituitary continues at the University of North Carolina.