A NATIONAL STABLE ISOTOPE RESOURCE AT LOS ALAMOS

我们建议继续运行国家稳定同位素 资源(SIR)作为洛杉矶更大的图标计划的一部分 阿拉莫斯国家实验室。图标程序，以 碳(12C，13C)、氧(16O，17O，18O)和氮的同位素 (14N、15N)，包括生产大量的 CO和NO低温蒸馏分离同位素 毒气。SIR的主要目标是促进 这些同位素在解决基本问题中的新应用 中国生物化学、生物物理学中的问题和实际问题 医学。该提案的研究部分表明 高效氨基酸特异性标记方法的建立 酸、核酸和酶辅助因子。通过制作这些 提供给研究界的材料，资源将 为蛋白质和核酸的基础研究做出贡献 结构、蛋白质-核酸相互作用和结构-- 血红素蛋白中的功能关系。 该资源将继续开展一系列协作活动 以及旨在扩大稳定的用途的服务活动 同位素在生物医学研究中的应用，这将有助于 通过发展稳定的同位素标记化合物的商业 设计有效的程序将标记的CO和NO转换为 有用的材料。 成为资源焦点的特殊化合物 活动的选择将与资源的 并将提供给外部调查人员 在竞争的基础上。这是最重要的服务之一 资源的一部分。

We propose to continue the operation of a national stable isotope resource (SIR) as part of the larger ICONs program at the Los Alamos National Laboratory. The ICONs program, named for the isotopes of carbon (12C, 13C), oxygen (16O, 17O, 18O), and nitrogen (14N, 15N), includes the production of large quantities of separated isotopes by low temperature distillation of CO and NO gases. The principal goal of the SIR is to promote the development of new application of these isotopes to solving fundamental problems in biochemistry, biophysics and practical problems in medical science. The research component of the proposal suggests development of efficient procedures for specific labeling of amino acids, nucleic acids, and enzyme cofactors. By making these materials available to the research community, the Resource will contribute to fundamental studies of protein and nucleic acid structure, protein-nucleic acid interactions, and structure- function relations in heme proteins. The Resource will continue to carry out a spectrum of collaborative and service activities aimed at broadening the uses for stable isotopes in biomedical research, and it will contribute to the developing commerce in stable isotopically labeled compounds by devising efficient procedures for converting labeled CO and NO to useful materials. The particular compounds which become the focus of Resource activities will be selected in consultation with the Resource's Advisory Committee and will be provided to external investigators on a competitive basis. This is one of the most important services of the Resource.