GENE AND TRANSGENE REGULATION IN THE DEVELOPING MOUSE

我们的实验室继续研究基因控制的机制。 在鼠标中，使用了各种方法。其中的第一个 关注不同的同源异型盒基因在 发育中的胚胎。我们发现Hox 1.1和Hox 1.5是 差异表达，并且它们的表达与 妊娠中期花纹形成的特定事件。另一个 我们的研究涉及癌基因的作用，一种 原癌基因和DNA复制因子分别在 转基因小鼠的晶状体。我们发现这些因素中的每一个 以一种不同的方式影响体内的细胞生长。我们还发现， 转基因介导的体内细胞转化是一种新的、 使高度专业化的细胞系统永垂不朽的强大方式 作为晶状体细胞。项目中解决人类问题的部分 艾滋病导致了一项意外的发现。所有单元格中 转基因小鼠中能够激活HIV LTR的系统， 皮肤的朗格汉斯细胞似乎最强大。这 强化巨噬细胞参与艾滋病的观点 发病机制。在另一项具有生物医学重要性的实验中，我们 已经表明转基因动物是人类的潜在来源 具有治疗价值的蛋白质。我们已经培育出了能够产生 他们的牛奶中含有大量的组织型纤溶酶原激活剂。这 蛋白质被用来治疗凝血障碍。决赛 这份报告涉及到小鼠的组织特异性表达 P450基因。Pl和P3，2基因表达的原位分析 不同的P450基因，已经指出了参与其中的特定细胞系统 在控制吸烟和其他环境污染物方面。

Our laboratory continues to investigate mechanisms of gene control in the mouse, using a variety of approaches. The first of these concerns the expression of distinct homeobox genes in the developing embryo. We find that Hox 1.1 and Hox 1.5 are differentially expressed and that their expression coincides with specific events of pattern formation during midgestation. Another of our studies deals with the action of an oncogene, a protooncogene and a DNA replication factor, respectively, in the lens of transgenic mice. We find that each of these factors affects cell growth in vivo in a distinct way. We also find that transgene mediated cell transformation in vivo is a new and powerful way of immortalizing highly specialized cell systems such as the lens cell. The part of the project that addresses the human AIDS disease has produced an unsuspected finding. Of all cell systems in the transgenic mouse able to activate the HIV LTR, the Langerhans cell of the skin appears the most powerful. This enforces the view of strong macrophage involvement in AIDS pathogenesis. In another experiment of biomedical importance, we have shown transgenic animals to be a potential source for human proteins of therapeutic value. We have generated mice that produce large amounts of tissue plasminogen activator in their milk. This protein is used for treatment of clotting disorders. The final study of this report concerns tissue specific expression of murine P450 genes. In situ analysis of expression of Pl and P3, two distinct P450 genes, has pointed out specific cell systems involved in the control of smoking and other environmental noxes.