IMAGE ANALYSIS AND ENHANCEMENT SYSTEM FOR NEUROBIOLOGY

电子和计算技术的快速发展 最近，它使收集和分析数据成为可能， 细胞的三维结构。 这些新技术正在 对神经生物学领域尤为重要， 神经元的三维复杂性以及 这种复杂性对神经系统功能的影响。 现在 可以获得准确、可重复的测量结果， 神经元复杂形状的重建。 的可用性 这项技术正在改变神经生物学家对 在成人和发育中的神经系统中， 系统，并大大有助于提高认识 这个三维空间的功能意义 复杂性 申请的设备有三个用途：1） 神经元树突树的三维重建 通过高尔基体法浸渍或用细胞内染料填充， 2)电子显微镜三维重建 获得连续切片，3）增强和数字化 用荧光染料染色的图像或通过 微分干涉对比度或其它光学增强 方法. 该设备或其他类似设备 本园区或相邻园区不提供这些功能 罗格斯大学校园。 本文件中要求的设备 应用程序将为不同的神经科学家群体提供 现代形态学的能力，是必不可少的， 当代神经生物学

Rapid technical developments in electronics and computing have recently made it possible to collect and analyze data concerning the three-dimensional structure of cells. These advances are particularly important to the field of neurobiology because of the three-dimensional complexity of neurons and the relationship of this complexity to the function of the nervous system. it is now possible to obtain accurate, reproducible measurements and reconstructions of the complex shape of neurons. The availability of this technology is changing the way neurobiologists conceive of cell-cell interactions in both the adult and developing nervous system and is contributing greatly to an improved understanding of the functional significance of this three-dimensional complexity. The equipment requested has three purposes: 1) three-dimensional reconstruction of the dendritic trees of neurons impregnated by the Golgi method or filled by an intracellular dye, 2) three-dimensional reconstruction of electron microscopically obtained serial sections, and 3) the enhancement and digitization of images stained with fluorescent dyes or visualized by differential interference contrast or other optical enhancement methods. This equipment or other equipment with similar capabilities is not available on this campus or on the adjoining campus of Rutgers University. The equipment requested in this application will provide a diverse group of neuroscientists with modern morphological capabilities that are essential to the contemporary neurobiology.