ANATOMICAL ANALYSIS OF NEURONAL CIRCUITS

以前对神经元树突结构的研究 主要依赖于有百年历史的高尔基方法， 将生物标记物注射到单个神经元中的技术 vivo. 然而，由于技术原因， 有效地将这些方法与其它神经解剖学方法联合收割机结合 标签技术。 最近推出的方法， 细胞内注射荧光染料，荧光黄， 组织切片能够提供完整的图片， 神经元的几何形状，并有潜力用于结合 其他神经解剖学技术。 这个项目涉及 细胞内注射荧光黄与 逆行标记技术和免疫组织化学， 目的是扩展神经元连接的分析， 发射机的特性比以前更精细。 首先要解决两个问题：（1）是否有一个点- 丘脑皮质轴突侧支之间的点相互作用， 支配丘脑网状核和 （2）在丘脑上的什么地方， 网状核神经元的树突做丘脑中央轴突 抵押品终止？

Previous studies of the dendritic structure of neurons have predominantly relied on the century-old Golgi methods and the technique of injecting biological markers into single neurons in vivo. For technical reasons, however, it has been difficult to effectively combine these methods with other neuroanatomical labeling techniques. The recently introduced method of intracellularly injecting a fluorescent dye, Lucifer yellow, in fixed tissue slices is capable of providing a complete picture of neuronal geometry and has the potential for use in conjunction with other neuroanatomical techniques. This project involves intracellular injection of Lucifer yellow in combination with retrograde labeling techniques and immunohistochemistry, and its objective is to extend the analysis of neuronal connectivity and transmitter specificities to a finer level than previously possible. Initially two questions will be addressed: (1) is there a point-to- point reciprosity between thalamocortical axon collaterals that innervate the thalamic reticular nucleus and the neurons in the nucleus which project back to the thalamus; and (2) where on the dendrites of retricular nucleus neurons do thalamocrotical axon collaterals terminate?