BIOLOGICAL MODIFICATION OF HUMAN GLIOMA CELLS IN VITRO

癌细胞表面的各种产物脱落 可能在粘连丧失、转移、 免疫阻断因子和其他病理生理因素的产生 癌症方面(Black PH，NEJM.303：1315,1980)。人类 胶质瘤细胞在组织培养中大量产生和脱落在培养液中 糖胺多聚糖(GAG)含量高于正常神经胶质细胞 细胞(Glimelius et al Biochem J.172：443,1978)。 糖胺聚糖是一种多阴离子化合物，通常结合在一起。 共价连接到蛋白质核心。它们是一个重要的组成部分 细胞表面，并与细胞间的相互作用有关。那里 也是透明质酸酶敏感的存在的证据 包括胶质瘤在内的一些肿瘤细胞系上的保护性涂层， 而且有人建议，保护性恶作剧外套可以 阻碍免疫反应和化疗的疗效。 我们的目标是改变或防止合成和脱落 在培养的人脑胶质瘤细胞中的GAG，并可能呈现 他们更容易受到化疗的影响 免疫反应。出于此目的，我们使用，单独或在 组合，分级剂二甲基亚砜 DMSO、丁酸钠、维甲酸和地塞米松。 已知这些试剂会影响其他生物体内GAG的合成 系统。

Shedding of various products from the cell surface of cancer cells may have an important role in loss of adhesion, metastasis, generation of immune-blocking factors and other pathophysiologic aspects of cancer (Black PH, NEJM. 303:1315, 1980). Human glioma cells in tissue culture produce and shed in the media much greater amounts of glycosaminoglycans (GAGs) than normal glial cells (Glimelius et al Biochem J. 172:443, 1978). Glycosaminoglycans are polyanionic compounds, usually bound covalently to a protein core. They are a prominent component of the cell surface and are implicated in cell-cell interaction. There is also evidence for existence of hyaluronidase-sensitive protective coats on some neoplastic cell lines including gliomas, and it has been suggested that the protective GAG coat may impede the immune response and the efficacy of chemotherapy. Our objective was to modify or prevent the synthesis and shedding of GAGs in human glioma cells in culture, and possibly to render them more susceptible to chemotherapy and more immunologically responsive. For this purpose we use, singly or in combination, the differentiating agents dimethyl sulfoxide (DMSO), sodium butyrate, retinoic acid, and dexamethasone. These agents are known to affect the synthesis of GAGs in other systems.