MOLECULAR BIOLOGY OF NEUROTRANSMITTER RECEPTORS

最近，我们发表了一篇关于克隆和序列分析的论文， 人脑β-肾上腺素能受体（FEBS Lett. 211：200 - 206，1987）。 在最近的一篇关于进化的评论中， 肾上腺素能和胆碱能受体，我们进一步假设， 毒蕈碱胆碱能受体已经存在了600多年， 百万年（Prog。神经生物学，药理学和 生化证据表明β-肾上腺素能受体 在进化后期出现， 是基因复制的结果。 为了进一步回答 关于受体进化的问题，我们正处于 克隆β-肾上腺素能和毒蕈碱胆碱能受体 从鲨鱼和果蝇的组织中分离出来 神经受体的克隆 基因也使我们能够研究结构与功能的关系 这些受体的基因突变。 我们有 成功表达人β 2肾上腺素能受体基因 是一种以前缺乏功能性受体的小鼠细胞系。 一 一系列单氨基酸取代突变体也已被发现。 正在制作中，正在进行鉴定。 研究 这些突变体应该能帮助我们定位那些重要的残基 负责配体结合、信号转导或维持 结构完整性。* （1987年7月从利比里亚国家警察转移）。

Recently, we published a paper on Cloning and Sequence Analysis of the Human Brain beta-Adrenergic Receptor (FEBS Lett. 211:200-206, 1987). In a recent review concerning the evolution of adrenergic and cholinergic receptors we further postulated that the muscarinic cholinergic receptor has existed for over 600 million years (Prog. Neurobiol., in press) Pharmacological and biochemical evidence suggests that the beta-adrenergic receptor appeared later in evolution raising the possibility that it evolved as a result of gene duplication. In order to further answer questions concerning receptor evoltion we are in the process of cloning beta-adrenergic and muscarinic cholinergic receptors from shark and Drosophila tissue. The cloning of neural receptor genes also allowed us to study the structure-function relationship of these receptors by site-directed mutagenesis. We have successfully expressed the human beta2-adrenergic receptor gene is a mouse cell line previously lacking the functional receptor. A series of single amino acid substitution mutants have also been made and are in the process of being characterized. Study of these mutants should help us to locate those important residues responsible for ligand binding, signal transduction or maintenance of structural integrity. *(Transferred from LNP on 7/87).