UW X-RAY DIFFRACTOMETER AND AREA DETECTOR

请拨资金用于X光快速数据收集设施 它将包括一个最先进的区域探测器和 一种新型的密封管源衍射仪。该设备将 是用于收集X射线的主要数据收集设备 氧化和还原形式的高分辨率衍射数据 蓝铜蛋白、铁硫蛋白、抗肿瘤蛋白 在有和没有DNA嵌入物的情况下，不同 氧结合蛋白杂氰菊酯的氧合形式 变构效应结合，硫代肌红蛋白，铁氧还蛋白还原酶， 在非自然宿主中表达的人类蛋白，以及诸如 三斜溶菌酶(用于测试X射线的精制技术 基于绕射的模型)。 通过减少收集数据所需的时间长度和 从而减少了数据收集中的严重系统错误 系统将以更高的分辨率加快结构研究 稳定的化合物，并使更大的分析成为可能 比我们以前研究过的大分子要多。

Funds are requested for a rapid data collection facility for X-ray diffraction which will include a state-of-the-art area detector and a new diffractometer with a sealed tube source. The device will be the primary data collection facility used to collect X-ray diffraction data at high resolution on oxidized and reduced forms of blue copper proteins, iron-sulfur proteins, antitumor proteins with and without DNA intercalators bound to them, different oxygenated forms of the oxygen binding protein hemerythrin with allosteric effectors bound, sulfmyoglobin, ferredoxin reductase, human proteins expressed in unnatural hosts, and proteins such as triclinic lysozyme (for testing of refinement techniques of X-ray diffraction based models). By reducing the length of time necessary to collect data and thereby reducing serious systematic errors in data collection the system will expedite structural studies at higher resolution of less stable compounds and make possible analysis of larger macromolecules than we have previously studied.