CULTURE PHYSIOLOGY AND ANTIGENIC ANALYSIS OF SEXUAL STAGES OF MALARIA PARASITES

本项目的目的是确定和分离编码P. 恶性疟原虫抗原是抗体的靶标， 这种寄生虫的配子体对蚊子的感染力。 该repetoire 特异性针对恶性疟原虫性期的单克隆抗体， 已被扩展以进一步定义靶抗原，所述靶抗原显示包括 230 kDa配子表面蛋白。 克隆编码 对于这些抗原， 免疫选择编码70 kDa热休克蛋白的DNA克隆， 似乎在结构上与230 kDa配子表面蛋白相关 也就是搜查的目标 实地研究表明，接触恶性疟原虫的人群 在巴布亚新几内亚的传播，针对230 kDa的 以及48 kDa和45 kDa配子表面蛋白。 间日疟原虫研究 在斯里兰卡发现，某些抗配子抗体 寄生虫在高浓度下对蚊子的感染力， 低浓度下的感染性。 具有特异性的人T细胞克隆 恶性疟原虫的配子抗原已经从 非免疫个体。 这些克隆具有"辅助" T细胞表型， 它们的反应性是组织相容性抗原限制的。 计划 使用这些克隆来确定配子抗原的T细胞表位。 对黑木耳无性系间杂交试验结果进行了分析。 几个实验室正在继续研究恶性疟原虫。 交叉杂交 会导致染色体大小的改变

The object of this project is to define and isolate genes coding P. falciparum antigens which are the targets of antibodies which block infectivity of gametocytes of this parasite to mosquitoes. The repetoire of monoclonal antibodies specific to sexual stages of P. falciparum has been expanded to further define target antigens which are shown to include the 230 kDa gamete surface protein of P. faciparum. Cloning genes coding for such antigens has been beset with problems including the immunoselection of DNA clones coding a 70 kDa heat shock protein which appears to be structurally related to the 230 kDa gamete surface protein which was the object of the search. Field studies have shown that human populations exposed to P. falciparum transmission in Papua New Guinea, elaborate antibodies against the 230 kDa and the 48 kDa and 45 kDa gamete surface proteins. Studies with P. vivax in Sri Lanka have shown that certain antigamete antibodies which supress infectivity of the parasite to mosquitoes at high concentrations, enhance infectivity at low concentrations. Human T-cell clones with specificity for P. falciparum gamete antigens have been isolated from the blood of non-immune individuals. These clones have "helper" T-cell phenotypes and their reactivity is histocompatibility antigen restricted. It is planned to use such clones to define the T-cell epitopes of gamete antigens. Analysis of the cross hybridization experiment between clones of P. falciparum is being continued in several laboratories. Cross hybridization has been shown to lead to changes in chromosome size.