STRUCTURE AND FUNCTION OF THE ADV GENOME

这个项目的目的是研究基因组的结构和功能。 水貂细小病毒(ADV)的阿留申病。约85%的 对减毒株ADV-G的DNA序列进行了推导 以M13为基础的双脱氧方法。研究结果表明，总体来说， 组织结构类似于其他无缺陷的细小动物，但 在核苷酸和预测的蛋白质水平上的同源性较低 而不是50%。代表15-88个MAP单位(MU)的分子克隆是 直接来自两个体内传代的强毒株和 与ADV-G相比。详细的限制性内切酶图谱显示这些病毒 是非常密切相关的，而且这三种病毒的片段 同样大小的。在大肠杆菌中表达这三种抗原的克隆 与病毒粒子、衣壳蛋白特异的血清反应，但尽管 DNA片段在长度上相同的事实是，蛋白质 由这两种体内病毒克隆编码的基因比 ADV-G。这一发现可能很重要，因为衣壳蛋白发现了 在来自强毒病毒的颗粒中也比 类似的ADV-G衣壳蛋白。它们之间的DNA序列比较 两种病毒和ADV-G正在进行中。

The purpose of this project is the study of genome structure and function of the Aleutian disease of mink parvovirus (ADV). Approximately 85% of the DNA sequence of the attenuated ADV-G strain has been deduced using the M13-based dideoxy methods. The findings suggest that the overall organization is similar to that of other nondefective parvoviurses, but that homology at both the nucleotide and predicted protein level is less than 50%. Molecular clones representing the 15-88 map units (MU) were derived directly from two in vivo passaged virulent ADV strains and compared to ADV-G. Detailed restriction mapping indicated that the viruses were very closely related and that the segments of all three viruses were the same size. Clones of all three expressed antigens in E. coli that reacted with sera specific for virion, capsid proteins, but, in spite of the fact that the DNA segments are identical in length, the proteins encoded by clones of the two in vivo viruses were 2-3 kd larger than those of ADV-G. This finding may be important because the capsid proteins found in particles from virulent viruses also are 2-3 kd larger than the comparable ADV-G capsid proteins. DNA sequence comparison between these two viruses and ADV-G is underway.