RAS P21 PROTEINS AND INDENTIFYING DBL ONCOGENE PRODUCT

项目1：在我们继续努力了解结构-功能的过程中 与ras p21的关系，我们对4-5个氨基酸进行了亚基缺失。 以p21蛋白作为抗ras p21单抗表位的研究 抗体，Y13-259。该抗体可逆转p21的转化作用。 并且还可以阻止DNA合成的启动。然而，Y13-259有 不会直接干扰p21的已知生化特性。这些 研究表明，这种抗体可能会影响一些尚未检测到的抗体。 P21的功能。深入了解Y13-259结合的功能 ，这些突变体目前正在分析它们的 生物化学和生物学特性。 项目2：从人弥漫性B细胞中分离出一种新的癌基因DB1 淋巴瘤，以前已经被证明可以赋予转化的表型 NIH/3T3细胞。我们目前的研究是为了努力 鉴定DB1癌基因产物并鉴定其部分特性 结构和功能特性。一个66,000道尔顿蛋白(P66) 在获得DB1癌基因的细胞中被鉴定，而不是 存在于由其他癌基因转化的细胞或未转化的NIH/3T3中 细胞。此外，确定P66由DB1编码 致癌基因。P66是一种磷酸蛋白，丝氨酸是P66的主要结合部位 磷酸化，根据其判断它是一种细胞质蛋白 亚细胞分布。

Project 1: In our continued effort to understand the structure-function relationship of ras p21, we have made inframe deletions of 4-5 amino acids in the region of p21 protein as the epitope for anti-ras p21 monoclonal antibody, Y13-259. This antibody can revert the transforming effect of p21 and can also block the initiation of DNA synthesis. However, Y13-259 does not directly interfere with known biochemical properties of p21. These studies suggest that this antibody may affect some as yet undetected function of p21. To gain insights into the function of Y13-259 binding region of p21, these mutants are currently being analyzed for their biochemical and biological properties. Project 2: A novel oncogene, db1, isolated from a human diffuse B-cell lymphoma, has been previously shown to confer the transformed phenotype on NIH/3T3 cells. Our present studies were undertaken in an effort to identify the db1 oncogene product and to characterize some of its structural and functional properties. A 66,000 dalton protein (p66) has been identified in cells that have acquired db1 oncogene, while it is not present in cells transformed by other oncogenes or in untransformed NIH/3T3 cells. Furthermore, it is established that p66 is encoded by db1 oncogene. p66 is a phosphoprotein with serine a major site of phosphorylation and it is a cytoplasmic protein as judged by its subcellular distribution.