SERUM-FREE CULTURE OF MOUSE KERATINOCYTES

建立了一种适用于BALB/MK小鼠的无血清限定培养体系 角质形成细胞系。结果发现，该细胞系只需要两个 生长因子，胰岛素和表皮生长因子(EGF)，用于生长速度 接近于在含血清的培养液中添加 和EGF一起。 然后，使用此定义的介质系统来确定准确的增长 不同基因转化的BALB/MK细胞对因子的要求 肿瘤病毒。结果发现，几乎所有的病毒转化子都是 能够在没有EGF的情况下生长。然而，不同的肿瘤病毒是不同的 在他们如何有效地消除对EGF的依赖上。H-Ras-、K-Ras-、FGR-和 ABL转化的细胞变得完全不依赖于EGF。Fms-、mos-和 ErbB转化的细胞在没有EGF的情况下生长，但在EGF的作用下生长得更好 当时在场。转化FGR和MOS的细胞也能在 表皮生长因子的缺失。 这种定义的媒体系统也已被改编为一种严格的 选择脱离以下生长因子要求的细胞 转型。

A serum-free defined media system was developed for the BALB/MK mouse kertinocyte cell line. It was found that this cell line required only two growth factors, insulin and epidermal growth factor (EGF), for growth rates which approached those achievable in serum-containing media supplemented with EGF. This defined media system was then used to determine the precise growth factor requirements of BALB/MK cells transformed with a variety of oncoviruses. It was found that nearly all of the viral transformants were able to grow in the absence of EGF. However, different oncoviruses varied in how efficiently they abrogated EGF dependence. H-ras-, K-ras-, fgr- and abl-transformed cells became entirely EGF-independent. Fms-, mos-, and erbB-transformed cells grew in the absence of EGF, but grew better when EGF was present. Cells transformed with fgr and mos were also able to grow in the absence of EGF. This defined media system has also been adapted as a method of stringently selecting for cells which escape from growth factor requirements following transformation.