BIOLOGICAL MODIFICATION OF HUMAN GLIOMA CELLS IN VITRO

癌细胞表面各种产物的脱落可能会对 在粘连丧失、转移、产生 免疫阻断因子和癌症的其他病理生理学方面。 人脑胶质瘤细胞在组织培养上清液中大量产生和脱落 糖胺多聚糖(GAG)的含量高于正常神经胶质细胞。 糖胺聚糖是一种多阴离子化合物，通常以共价方式与 蛋白质核心。它们是细胞表面的重要组成部分，是 牵涉到细胞与细胞的相互作用。也有证据表明存在 某些肿瘤细胞系透明质酸酶敏感保护膜的研究 包括神经胶质瘤，而且有人建议保护性的恶作剧外套 可能会阻碍免疫反应和化疗的疗效。我们的 目的是改变或防止GAG的合成和脱落 培养中的人脑胶质瘤细胞，并可能使其更容易受到影响 对化疗和更多的免疫反应。为此，我们 单独使用或联合使用，区分剂二甲基亚砜 DMSO、丁酸钠、维甲酸和地塞米松。这些特工 已知会影响其他系统中GAG的合成。

Shedding of various products from the cell surface of cancer cells may have an important role in loss of adhesion, metastasis, generation of immune-blocking factors and other pathophysiologic aspects of cancer. Human glioma cells in tissue culture produce and shed in the media much greater amounts of glycosaminoglycans (GAGs) than normal glial cells. Glycosaminoglycans are polyanionic compounds, usually bound covalently to a protein core. They are a prominent component of the cell surface and are implicated in cell-cell interaction. There is also evidence for existence of hyaluronidase-sensitive protective coats on some neoplastic cell lines including gliomas, and it has been suggested that the protective GAG coat may impede the immune response and the efficacy of chemotherapy. Our objective was to modify or prevent the synthesis and shedding of GAGs in human glioma cells in culture, and possibly to render them more susceptible to chemotherapy and more immunologically responsive. For this purpose we use singly or in combination, the differentiating agents dimethyl sulfoxide (DMSO), sodium butyrate, retinoic acid, and dexamethasone. These agents are known to affect the synthesis of GAGs in other systems.