DELETION MUTANTS IN CONTROL REGIONS THAT REGULATE SV40 TRANSCRIPTION

在大多数真核基因中，以前已经发现了一组共同的核苷酸 RNA转录的起始点。这些核苷酸，称为 无论是塔塔还是Goldberg-Hogness盒子，都是 体内和体外转录。晚些时候SV40的控制区 转录本已经通过定点突变和 缺失突变的产生。增强或抑制基因突变的基因 已经确定了单个起始点的转录活性，并 不同于共识的Goldberg-Hogness盒子。当这个上行晚了 转录控制区被转换成类似于 TATA BOX目前在SV40早期的启动子中，有最强的 体外增强晚期转录。所需GC之间的间距 Rich结构域和TATA影响早期转录水平 这表明在RNA聚合酶II中，在这些地方同时存在接触 两个地点。

In most eucaryotic genes, a common set of nucleotides has been found before the start site of RNA transcription. These nucleotides, referred to as either a TATA or Goldberg-Hogness box, are important determinants of transcription in vivo and in vitro. The control region for the late SV40 transcripts have been examined by site specific mutagenesis and by generation of deletion mutations. Mutations that enhance or suppress the transcriptional activity of a single start site have been identified and differ from the consensus Goldberg-Hogness box. When this upstream late transcription control region is converted to a sequence that is similar to the TATA box present in the SV40 early promoter, there is the strongest enhancement of late transcription in vitro. Spacing between a required GC rich domain and the TATA effects the level of early transcription suggesting that there is simultaneous contact in RNA polymerase II at these two sites.