A MFBS SCOR AT THE MOUNT DESERT ISLAND BIOLOGICAL LAB

海洋与淡水生物医学科学专业研究中心 它将利用行政部门的组成部分， 山的设施和科学工作人员。荒岛生物 实验室。MFBS SCOR将提供行政和技术支持 援助、实验动物、用品和资本设备 促进生物医学的互动、协作和培训 研究有毒物质的科学家(包括临床医生和生理学家) 重金属对膜功能的影响。此外，它还将提供 用于快速传播从这些研究得出的数据。动物 将使用同时模拟被动和主动膜传输的系统 人体肾脏、肠道和肝脏的突起 上皮--已知对重金属毒性敏感的组织。 具体地说，研究小组将研究重金属对以下方面的影响： 1.氨、氢和HCO3通过鱼鳃的运输；2.鱼鳃的运输 K由鱼膀胱；3.葡萄糖和氨基酸的运输 牙鲨肾脏微囊的跨膜转运和氯化钠/氯化钾的跨膜共转运 鲨鱼直肠腺微囊；4.氯化钠/氯化钾跨离体膜转运 直肠腺小管；5.维持细胞内pH、钙浓度、 和膜PD在分离的直肠腺细胞中；6.细胞工作 分离、灌流和体外直肠腺；7.氨基酸的转运 横跨鱼肠；8.直肠腺细胞的容量调节； 激素刺激氯转运的刺激/反应耦合，以及 鱼鳃的血管活性；10.钠、钾和氯的跨膜转运途径 鱼的膀胱；11.八脚鳗肾小球的通透性 肾脏；12.钠和氯在鱼鳃上的运输；13.鱼的运输 钠和氯通过鱼的角膜；以及，14.鱼的运输方式 甲基汞穿过肝管上皮。 建议这个MFBS SCOR将提供一个独特的环境 当前和未来生物医学科学家的研究和培养 对毒性效应的细胞和亚细胞机制感兴趣 重金属。

A Marine and Freshwater Biomedical Sciences Specialized Center of Research is proposed which will utilize components of the administration, facilities, and scientific staff of the Mt. Desert Island Biological Laboratory. The MFBS SCOR will provide the administrative and technical assistance, experimental animals, supplies, and capital equipment to promote the interaction, collaboration, and training of biomedical scientists (both clinicians and physiologists) investigating the toxic effects of heavy metals on membrane function. In addition, it will provide for the rapid dissemination of data derived from these studies. Animal systems will be used which model both passive and active membrane transport processes found in the human renal, intestinal and hepatic epithelia--tissues with known sensitivity to heavy metal toxicity. Specifically, research groups will examine the effects of heavy metals on: 1. Transport of ammonia, H, and HCO3 across the fish gill; 2. Transport of K by the fish urinary bladder; 3. Transport of glucose and amino acids across flounder renal microvesicles, and cotransport of NaCl/KC1 across shark rectal gland microvesicles; 4. Transport of NaCl/KC1 across isolated rectal gland tubules; 5. Maintenance of intracellular pH, Ca concentration, and membrane PD in isolated rectal gland cells; 6. Cellular work of isolated, perfused, and in vitro rectal gland; 7. Transport of amino acids across the fish gut; 8. Volume regulation by rectal gland cells; 9. Stimulus/response coupling of hormonal stimulation of transport of Cl, and vasoactivity, of fish gills; 10. Transport pathways for Na, K and Cl across the fish urinary bladder; 11. Glomerular permeability of the hagfish kidney; 12. Transport of Na and Cl across the fish gill; 13. Transport of Na and Cl across the fish cornea; as well as, 14. The mode of transport of methlymercury across the hepatic duct epithelium. It is proposed that this MFBS SCOR will provide a unique milieu for research and training of present and future biomedical scientists interested in the cellular and sub-cellular mechanisms of the toxic effects of heavy metals.