DETERMINATION OF TOTAL PROTEIN BY USING PURIFIED HUMAN SERUM PROTEIN FRACTIONS

四种常用下限测定方法的比例偏差 用人类蛋白质组分(Cohn)评估蛋白质浓度 组分II、III、IV和V)。每一部分都被分配了一种蛋白质 浓缩值是用缩二脲方法测定的 纯化的人血清白蛋白单体建立定标。全 四种方法(考马斯蓝染料结合，Lowry等人，紫外线 吸收和免疫比浊法)给出了可接受的结果 组分V(白蛋白)。紫外吸收和Lowry方法 高估了三种球蛋白组份(II、III和IV)和 考马斯蓝染料结合及免疫比浊法 低估了这些球蛋白组分。的最小比例偏差 球蛋白分级采用Lowry法。

The proportional bias of four methods frequently used for determining low concentrations of protein was evaluated with human protein fractions (Cohn Fractions II, III, IV and V). Each fraction was assigned a protein concentration value which was determined by the biuret method using purified human serum albumin monomer to establish the calibration. All four methods (Coomassie blue dye-binding, Lowry et al., ultraviolet absorption, and the immunoephelometric) gave acceptable results for fraction V (albumin). The ultraviolet absorption and the Lowry methods overestimated the three globulin fractions (II, III, and IV) and the Coomassie blue dye-binding and the immunoephelometric methods underestimated these globulin fractions. The least proportional bias for the globulin fractions was with the Lowry method.