PERTUSSIS HEAT LABILE TOXIN (HLT)--ISOLATION AND CHARACTERIZATION

百日咳杆菌产生几种蛋白质毒素， 毒素和皮肤坏死毒素（热不稳定毒素或HLT）。 HLT，注射 皮下注射到乳鼠中， 损伤。 这种毒素引起的损伤的程度、定位和性质 提示HLT参与了B的发病机制。百日咳。 条件 使用乳鼠模型进行毒素测定， 确立了习 通过使用常规的蛋白质纯化技术和 高压液相色谱法，HLT的均相制剂具有 得出了 这些研究表明，该毒素是一种单一的多肽 分子量约为150，000的链。 已对纯化的毒素进行氨基酸分析， 用来制造抗体 这些抗体被用来记录 目前毒素制剂的纯度。 此外，HLT抗体 获得了中和活性和高滴度血清（1/20，000 免疫兔子。 HLT除了介导皮肤坏死外， 病变，显示是致命的，诱导短期升高（3天）， 白色血细胞，并引起和显着萎缩和“漂白”的 脾脏 这些变化对可能的生物化学的意义 作用机制及其与百日咳发病机制的关系 正在接受调查

Bordetella pertussis produces several protein toxins including pertussis toxin and dermonecrotic toxin (heat labile toxin or HLT). HLT, injected subcutaneously into suckling mice, induces a lcoal pronounced hemorrhagic lesion. The extent, localization, and nature of this toxin-induced injury suggests HLT contributes to te pathogenesis of B. pertussis. Conditions for assay of the toxin using the suckling mouse model have been established. By use of conventional protein purification techniques and high pressure liquid chromatography, homogeneous preparations of HLT have been obtained. These studies show that the toxin is a single polypeptide chain with a molecular weight of about 150,000. The purified toxin has been subjected to amino acid analysis and has been used to produce antibodies. These antibodies have been used to document the purity of current toxin preparation. In addition, HLT antibodies neutralize toxic activity and high titer sera (1/20,000) have been obtained from immunized rabbits. HLT, in addition to mediating a dermonecrotic lesion, was shown to be lethal, induce a short term elevation (3 days) of white blood cells, and elicit and marked atrophy and "bleaching" of the spleen. The significance of these changes to possible biochemical mechanisms of action and their relation to the pathogenesis of pertussis are being investigated.